Ser86

Site Information
TKNGLPGsRPGsPER SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448826

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 22 ) , mass spectrometry ( 3 , 4 , 5 , 6 , 7 , 9 , 10 , 11 , 14 , 16 , 17 , 18 , 19 , 20 , 22 ) , mutation of modification site ( 2 , 13 , 15 , 21 , 22 ) , phospho-antibody ( 2 , 13 , 15 ) , western blotting ( 2 , 13 , 15 )
Disease tissue studied:	bone cancer ( 15 ) , breast cancer ( 5 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 ) , colorectal cancer ( 13 ) , colorectal carcinoma ( 13 ) , leukemia ( 14 ) , acute myelogenous leukemia ( 14 ) , lung cancer ( 10 ) , non-small cell lung cancer ( 10 ) , melanoma skin cancer ( 6 )
Relevant cell line - cell type - tissue:	293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 18 ) , 293 (epithelial) [AT1 (human), transfection] ( 17 ) , 293 (epithelial) ( 21 ) , BaF3 ('B lymphocyte, precursor') ( 15 ) , breast ( 3 ) , BT-474 (breast cell) ( 5 ) , ES (stem) ( 21 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 11 ) , Flp-In T-Rex-293 (epithelial) ( 11 ) , H2009 (pulmonary) ( 10 ) , H2077 (pulmonary) ( 10 ) , H2887 (pulmonary) ( 10 ) , H322M (pulmonary) ( 10 ) , HCT116 (intestinal) ( 13 ) , HEK293T (epithelial) ( 15 ) , HeLa (cervical) ( 7 , 9 , 16 , 19 , 22 ) , Jurkat (T lymphocyte) ( 20 ) , K562 (erythroid) ( 9 ) , KG-1 (myeloid) ( 14 ) , MCF-7 (breast cell) ( 4 , 5 ) , MEF (fibroblast) ( 2 ) , Sf21 ( 22 ) , U2OS (bone cell) ( 15 ) , WM239A (melanocyte) ( 6 )

Upstream Regulation
Regulatory protein:	APP (human) ( 21 ) , Fe65 (human) ( 21 )
Putative in vivo kinases:	CDK1 (human) ( 21 ) , GSK3B (human) ( 13 , 15 )
Kinases, in vitro:	CDK1 (human) ( 13 ) , GSK3B (human) ( 13 , 15 )
Treatments:	Akt_inhibitor_IV ( 13 ) , etoposide ( 2 ) , metformin ( 4 ) , rapamycin ( 13 ) , RO-3306 ( 2 ) , SB216763 ( 1 ) , seliciclib ( 21 ) , serum_starvation ( 13 ) , siRNA ( 1 ) , SNS-032 ( 2 )

Downstream Regulation
Effects of modification on Tip60:	acetylation ( 15 ) , activity, induced ( 1 ) , protein stabilization ( 21 )
Effects of modification on biological processes:	apoptosis, induced ( 15 ) , DNA repair, induced ( 2 ) , transcription, altered ( 21 ) , transcription, induced ( 15 )

References
1	Cheng X, et al. (2019) Pacer Is a Mediator of mTORC1 and GSK3-TIP60 Signaling in Regulation of Autophagosome Maturation and Lipid Metabolism. Mol Cell 73, 788-802.e7 30704899 Curated Info
2	Li ML, et al. (2019) Phosphorylation of TIP60 Suppresses 53BP1 Localization at DNA Damage Sites. Mol Cell Biol 39 30297459 Curated Info
3	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
4	Sacco F, et al. (2016) Deep Proteomics of Breast Cancer Cells Reveals that Metformin Rewires Signaling Networks Away from a Pro-growth State. Cell Syst 2, 159-71 27135362 Curated Info
5	Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290 27362937 Curated Info
6	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
7	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
8	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
9	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
10	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
11	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
12	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
13	Lin SY, et al. (2012) GSK3-TIP60-ULK1 signaling pathway links growth factor deprivation to autophagy. Science 336, 477-81 22539723 Curated Info
14	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
15	Charvet C, et al. (2011) Phosphorylation of Tip60 by GSK-3 determines the induction of PUMA and apoptosis by p53. Mol Cell 42, 584-96 21658600 Curated Info
16	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
17	Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304 20686112 Curated Info
18	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
19	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
20	Rush J (2005) CST Curation Set: 918; Year: 2005; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XX[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) Akt Substrate Antibody Cat#: 9611 Curated Info
21	Hass MR, Yankner BA (2005) A {gamma}-secretase-independent mechanism of signal transduction by the amyloid precursor protein. J Biol Chem 280, 36895-904 16103124 Curated Info
22	Lemercier C, et al. (2003) Tip60 acetyltransferase activity is controlled by phosphorylation. J Biol Chem 278, 4713-8 12468530 Curated Info