Tyr515
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Home > Phosphorylation Site Page: > Tyr515  -  TrkB (mouse)

Site Information
PVIENPQyFGITNSQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448813
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 7 ) , mass spectrometry ( 6 , 8 , 9 ) , mutation of modification site ( 12 , 13 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 7 , 10 , 11 , 13 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 7 , 11 , 13 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 11 ) , 'brain, embryonic' ( 9 ) , 'brain, hippocampus' ( 1 ) , 'neuron, cochlear' ( 13 ) , 'neuron, cortical' ( 2 ) , 'neuron, cortical'-brain ( 3 , 4 , 7 , 13 ) , 3T3 (fibroblast) ( 13 ) , brain ( 6 , 8 ) , HEK293T (epithelial) ( 2 , 4 ) , MG87 (fibroblast) ( 2 ) , neuron-'brain, cerebral cortex' ( 5 )

Upstream Regulation
Putative in vivo kinases:
TrkB (mouse) ( 13 )
Putative upstream phosphatases:
PTPRA (human) ( 3 )
Treatments:
1Na-PP1 ( 7 ) , BDNF ( 2 , 4 , 7 , 10 , 13 ) , bicuculline ( 4 , 5 ) , Ca(2+) ( 10 ) , electrical_stimulation ( 10 ) , glycine ( 4 ) , L-NAME ( 5 ) , morphine ( 11 ) , PP1 ( 7 ) , siRNA ( 3 ) , Zn(2+) ( 7 )

Downstream Regulation
Effects of modification on TrkB:
enzymatic activity, induced ( 3 , 13 ) , phosphorylation ( 13 ) , receptor internalization, altered ( 10 )
Effects of modification on biological processes:
cell differentiation, induced ( 3 ) , cell growth, altered ( 13 ) , cell growth, inhibited ( 3 ) , neural plasticity ( 5 ) , signaling pathway regulation ( 3 )

References 

1

Zhu ZA, et al. (2023) CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling. Cell Rep 42, 113202
37777961   Curated Info

2

Casarotto PC, et al. (2021) Antidepressant drugs act by directly binding to TRKB neurotrophin receptors. Cell 184, 1299-1313.e19
33606976   Curated Info

3

Tomita H, et al. (2020) The Protein Tyrosine Phosphatase Receptor Delta Regulates Developmental Neurogenesis. Cell Rep 30, 215-228.e5
31914388   Curated Info

4

Lai KO, et al. (2012) TrkB phosphorylation by Cdk5 is required for activity-dependent structural plasticity and spatial memory. Nat Neurosci 15, 1506-15
23064382   Curated Info

5

Gallo EF, Iadecola C (2011) Neuronal nitric oxide contributes to neuroplasticity-associated protein expression through cGMP, protein kinase G, and extracellular signal-regulated kinase. J Neurosci 31, 6947-55
21562256   Curated Info

6

Wiƛniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

7

Huang YZ, McNamara JO (2010) Mutual regulation of Src family kinases and the neurotrophin receptor TrkB. J Biol Chem 285, 8207-17
20064930   Curated Info

8

Ballif BA, Carey GR, Sunyaev SR, Gygi SP (2008) Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain. J Proteome Res 7, 311-8
18034455   Curated Info

9

Guo A (2007) CST Curation Set: 2672; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Du J, et al. (2003) Regulation of TrkB receptor tyrosine kinase and its internalization by neuronal activity and Ca2+ influx. J Cell Biol 163, 385-95
14581459   Curated Info

11

Lucas G, et al. (2003) Neurotrophin-4 mediated TrkB activation reinforces morphine-induced analgesia. Nat Neurosci 6, 221-2
12601381   Curated Info

12

He XP, Minichiello L, Klein R, McNamara JO (2002) Immunohistochemical evidence of seizure-induced activation of trkB receptors in the mossy fiber pathway of adult mouse hippocampus. J Neurosci 22, 7502-8
12196573   Curated Info

13

Postigo A, et al. (2002) Distinct requirements for TrkB and TrkC signaling in target innervation by sensory neurons. Genes Dev 16, 633-45
11877382   Curated Info