Ser222
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Home > Phosphorylation Site Page: > Ser222  -  ACC2 (human)

Site Information
PtMRPsMsGLHLVKR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448808

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 15 ) , mass spectrometry ( 1 , 5 , 7 ) , phospho-antibody ( 2 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ) , western blotting ( 2 , 8 , 9 , 10 , 11 , 13 , 14 , 15 , 16 , 19 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 ) , hepatocellular carcinoma, surrounding tissue ( 7 ) , type 2 diabetes ( 2 ) , McArdle's disease ( 19 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 2 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ) , adipose tissue ( 12 ) , breast ( 1 ) , HAEC (endothelial) ( 9 ) , hepatocyte-liver ( 7 ) , liver ( 5 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 9 )
Treatments:
exercise ( 2 , 8 , 10 , 11 , 12 , 13 , 14 , 16 , 17 , 18 , 19 ) , exercise training ( 13 ) , H-CHO ( 14 ) , insulin ( 11 , 15 ) , L-CHO ( 14 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Kjøbsted R, et al. (2016) Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise. Diabetes 65, 1219-30
26822091   Curated Info

3

Kalsen A, et al. (2014) Effect of inhaled terbutaline on substrate utilization and 300-kcal time trial performance. J Appl Physiol (1985) 117, 1180-7
25257871   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

6

Treebak JT, et al. (2014) Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol 592, 351-75
24247980   Curated Info

7

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

8

Rose AJ, et al. (2009) Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type. Am J Physiol Regul Integr Comp Physiol 296, R326-33
19036825   Curated Info

9

Reihill JA, Ewart MA, Hardie DG, Salt IP (2007) AMP-activated protein kinase mediates VEGF-stimulated endothelial NO production. Biochem Biophys Res Commun 354, 1084-8
17276402   Curated Info

10

Roepstorff C, et al. (2006) Higher skeletal muscle alpha2AMPK activation and lower energy charge and fat oxidation in men than in women during submaximal exercise. J Physiol 574, 125-38
16600998   Curated Info

11

Kuhl JE, et al. (2006) Exercise training decreases the concentration of malonyl-CoA and increases the expression and activity of malonyl-CoA decarboxylase in human muscle. Am J Physiol Endocrinol Metab 290, E1296-303
16434556   Curated Info

12

Watt MJ, et al. (2006) Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue. Am J Physiol Endocrinol Metab 290, E500-8
16188906   Curated Info

13

McConell GK, et al. (2005) Short-term exercise training in humans reduces AMPK signalling during prolonged exercise independent of muscle glycogen. J Physiol 568, 665-76
16051629   Curated Info

14

Roepstorff C, et al. (2005) Malonyl-CoA and carnitine in regulation of fat oxidation in human skeletal muscle during exercise. Am J Physiol Endocrinol Metab 288, E133-42
15383373   Curated Info

15

Højlund K, et al. (2004) AMPK activity and isoform protein expression are similar in muscle of obese subjects with and without type 2 diabetes. Am J Physiol Endocrinol Metab 286, E239-44
14532170   Curated Info

16

Wojtaszewski JF, et al. (2003) Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle. Am J Physiol Endocrinol Metab 284, E813-22
12488245   Curated Info

17

Nielsen JN, et al. (2003) 5'-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle. J Appl Physiol 94, 631-41
12391032   Curated Info

18

Wojtaszewski JF, et al. (2002) Dissociation of AMPK activity and ACCbeta phosphorylation in human muscle during prolonged exercise. Biochem Biophys Res Commun 298, 309-16
12413941   Curated Info

19

Nielsen JN, et al. (2002) Role of 5'AMP-activated protein kinase in glycogen synthase activity and glucose utilization: insights from patients with McArdle's disease. J Physiol 541, 979-89
12068056   Curated Info