Thr1466
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Home > Phosphorylation Site Page: > Thr1466  -  TSC2 (rat)

Site Information
GLRPRGYtISDSAPS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448546

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 13 ) , immunoprecipitation ( 5 , 9 , 13 ) , mutation of modification site ( 13 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 ) , phosphopeptide mapping ( 13 ) , western blotting ( 2 , 4 , 5 , 6 , 7 , 8 , 12 )
Disease tissue studied:
breast cancer ( 6 ) , liver cancer ( 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 2 , 8 , 9 , 10 ) , 293 (epithelial) ( 11 ) , 293 (epithelial) [TSC2 iso2 (rat)] ( 13 ) , heart ( 7 ) , L6 (myoblast) ( 4 ) , liver ( 6 ) , mammary gland ( 6 ) , myocyte-heart ( 5 , 11 ) , RIN (epithelial) ( 12 )

Upstream Regulation
Regulatory protein:
FAK (rat) ( 5 ) , SHP-2 (rat) ( 5 )
Kinases, in vitro:
Akt1 (human) ( 11 , 13 ) , p90RSK (human) ( 11 )
Treatments:
API-2 ( 4 ) , cyclic_stretch ( 5 ) , development ( 2 ) , food deprivation ( 6 ) , glucosamine ( 12 ) , IGF-1 ( 8 ) , indinavir ( 10 ) , insulin ( 3 , 4 , 10 , 11 , 12 ) , Intralipid ( 8 ) , LY294002 ( 11 ) , meal feeding ( 7 , 9 ) , paracetamol ( 2 ) , phenylephrine ( 11 ) , phorbol_ester ( 11 ) , PIK90 ( 4 ) , PP2 ( 5 ) , PP242 ( 4 ) , rapamycin ( 4 ) , U0126 ( 11 )

References 

1

Lee Hamilton D, et al. (2014) Molecular brakes regulating mTORC1 activation in skeletal muscle following synergist ablation. Am J Physiol Endocrinol Metab 307, E365-73
24961241   Curated Info

2

Wu M, et al. (2010) Acetaminophen improves protein translational signaling in aged skeletal muscle. Rejuvenation Res 13, 571-9
20818934   Curated Info

3

Blancquaert S, et al. (2010) cAMP-dependent activation of mammalian target of rapamycin (mTOR) in thyroid cells. Implication in mitogenesis and activation of CDK4. Mol Endocrinol 24, 1453-68
20484410   Curated Info

4

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

5

Marin TM, et al. (2008) Shp2 negatively regulates growth in cardiomyocytes by controlling focal adhesion kinase/Src and mTOR pathways. Circ Res 103, 813-24
18757826   Curated Info

6

Jiang W, Zhu Z, Thompson HJ (2008) Dietary energy restriction modulates the activity of AMP-activated protein kinase, Akt, and mammalian target of rapamycin in mammary carcinomas, mammary gland, and liver. Cancer Res 68, 5492-9
18593953   Curated Info

7

Vary TC, Lynch CJ (2006) Meal feeding stimulates phosphorylation of multiple effector proteins regulating protein synthetic processes in rat hearts. J Nutr 136, 2284-90
16920842   Curated Info

8

Lang CH (2006) Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle. Am J Physiol Endocrinol Metab 291, E666-74
16684854   Curated Info

9

Vary TC, Lynch CJ (2006) Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation. Am J Physiol Endocrinol Metab 290, E631-42
16263769   Curated Info

10

Hong-Brown LQ, et al. (2005) Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle. Am J Physiol Endocrinol Metab 289, E382-90
15827064   Curated Info

11

Rolfe M, McLeod LE, Pratt PF, Proud CG (2005) Activation of protein synthesis in cardiomyocytes by the hypertrophic agent phenylephrine requires the activation of ERK and involves phosphorylation of tuberous sclerosis complex 2 (TSC2). Biochem J 388, 973-84
15757502   Curated Info

12

Andreozzi F, et al. (2004) Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells. Endocrinology 145, 2845-57
15001544   Curated Info

13

Inoki K, et al. (2002) TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling. Nat Cell Biol 4, 648-57
12172553   Curated Info