Ser876
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Home > Phosphorylation Site Page: > Ser876  -  PRKD2 (human)

Site Information
QGLAERIsVL_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448800

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 12 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , phospho-antibody ( 11 , 12 , 13 ) , western blotting ( 11 , 12 )
Disease tissue studied:
breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 13 ) , A498 (renal) ( 7 ) , breast ( 1 , 3 ) , HCT116 (intestinal) ( 9 ) , HeLa (cervical) ( 6 , 13 ) , HeLa S3 (cervical) ( 10 ) , HL60 (myeloid) ( 13 ) , Jurkat (T lymphocyte) ( 5 , 8 ) , MDA-MB-435S (breast cell) ( 9 ) , MV4-11 (macrophage) ( 9 ) , NCM460 (epithelial) ( 11 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 4 )

Upstream Regulation
Putative in vivo kinases:
PKCA (human) ( 12 ) , PKCE (human) ( 12 ) , PKCH (human) ( 12 )
Kinases, in vitro:
PRKD2 (human) ( 13 )
Treatments:
ET18OCH3 ( 12 ) , gastrin ( 12 ) , GF109203X ( 11 , 12 ) , LPA ( 11 ) , phorbol_ester ( 11 , 12 , 13 ) , Ro31-8220 ( 11 , 12 )

Downstream Regulation
Effects of modification on PRKD2:
enzymatic activity, induced ( 12 , 13 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
24117733   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

7

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

8

Possemato A (2010) CST Curation Set: 9348; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)Xp[ST](I/L/V)
Curated Info

9

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

10

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

11

Chiu TT, et al. (2007) Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-kappaB. Am J Physiol Cell Physiol 292, C767-77
16928771   Curated Info

12

Sturany S, et al. (2002) Mechanism of activation of protein kinase D2(PKD2) by the CCK(B)/gastrin receptor. J Biol Chem 277, 29431-6
12058027   Curated Info

13

Sturany S, et al. (2001) Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases. J Biol Chem 276, 3310-8
11062248   Curated Info

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