Ser1987
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Site Information
SPTFEEGsQGTTISS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448797

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 25 ) , immunoprecipitation ( 25 ) , mass spectrometry ( 6 , 7 , 8 , 9 , 12 , 14 ) , mutation of modification site ( 18 , 27 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 10 , 11 , 13 , 15 , 16 , 17 , 18 , 20 , 21 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ) , western blotting ( 2 , 3 , 4 , 5 , 10 , 11 , 13 , 15 , 16 , 17 , 20 , 21 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 32 )
Disease tissue studied:
bone cancer ( 28 ) , leukemia ( 5 , 8 , 17 ) , acute myelogenous leukemia ( 8 ) , chronic lymphocytic leukemia ( 5 ) , chronic myelogenous leukemia ( 17 ) , neuroblastoma ( 16 ) , melanoma skin cancer ( 12 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 6 ) , 'brain, embryonic' ( 15 ) , 'hematopoietic progenitor, CD34+'-blood ( 17 ) , 293 (epithelial) ( 25 ) , B lymphocyte ( 27 ) , B lymphocyte-spleen ( 18 ) , B lymphocyte-spleen [NBS1 (human), transgenic] ( 24 ) , B16 (melanocyte) ( 12 ) , BaF3 ('B lymphocyte, precursor') [Abl (mouse), homozygous knockout] ( 17 ) , blood ( 8 ) , brain ( 9 ) , C2C12 (myoblast) ( 10 ) , embryo ( 9 ) , fibroblast ( 26 , 27 ) , HEK293T (epithelial) ( 2 ) , HeLa (cervical) ( 23 , 25 ) , hematopoietic progenitor-bone marrow ( 31 ) , L1210 (lymphoblast) ( 5 ) , liver ( 9 ) , lymphoblastoid ( 26 ) , MEF (fibroblast) ( 4 , 7 , 13 , 23 , 26 , 28 , 29 ) , MEF (fibroblast) [ATM (mouse)] ( 25 ) , MEF (fibroblast) [HMGA1 (mouse), homozygous knockout] ( 11 ) , MEF (fibroblast) [HMGA2 (mouse), homozygous knockout] ( 11 ) , MEF (fibroblast) [IGF1R (mouse)] ( 30 ) , MEF (fibroblast) [PTPN2 (mouse), homozygous knockout] ( 20 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 7 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 7 ) , Neuro-2a (neuron) ( 16 ) , neuron ( 16 ) , Saos-2 (bone cell) ( 28 ) , SL13 (fibroblast) ( 2 ) , spermatocytes ( 32 ) , testis ( 14 , 32 ) , thymocyte ( 21 )

Upstream Regulation
Regulatory protein:
53BP1 (mouse) ( 26 ) , Abl (mouse) ( 13 , 17 ) , ALKBH8 (mouse) ( 4 ) , ATM (mouse) ( 10 ) , H2AX (mouse) ( 26 ) , HBpX (retrovirus) ( 19 ) , NCK1 (mouse) ( 23 ) , PPM1D (mouse) ( 28 ) , PTPN2 (human) ( 20 ) , SMO (mouse) ( 22 ) , TSC2 (mouse) ( 29 )
Putative in vivo kinases:
ATM (mouse) ( 5 , 18 )
Kinases, in vitro:
ATM (mouse) ( 5 )
Putative upstream phosphatases:
PPM1D (mouse) ( 28 )
Treatments:
2-AP ( 31 ) , adriamycin ( 13 , 19 ) , aphidicolin ( 20 ) , cisplatin ( 17 ) , differentiation ( 3 ) , etoposide ( 15 ) , glucose_starvation ( 10 ) , H2O2 ( 4 ) , hydroxyurea ( 26 ) , hypoxia/reoxygenation ( 31 ) , imatinib ( 17 ) , ionizing_radiation ( 5 , 16 , 18 , 21 , 24 , 27 , 28 , 32 ) , KU-55933 ( 5 , 10 , 17 , 25 ) , LY294002 ( 17 ) , MMS ( 29 ) , NU7441 ( 5 ) , olaparib ( 2 ) , rotenone ( 4 ) , siRNA ( 25 ) , thymidine ( 20 ) , UV ( 23 , 25 , 26 , 28 )

Downstream Regulation
Effects of modification on ATM:
enzymatic activity, induced ( 5 ) , intracellular localization ( 25 , 27 )
Effects of modification on biological processes:
chromatin organization, altered ( 18 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Lee SS, et al. (2015) ATM Kinase Is Required for Telomere Elongation in Mouse and Human Cells. Cell Rep 13, 1623-32
26586427   Curated Info

3

Takagi M, et al. (2015) ATM Regulates Adipocyte Differentiation and Contributes to Glucose Homeostasis. Cell Rep
25683718   Curated Info

4

Endres L, et al. (2015) Alkbh8 Regulates Selenocysteine-Protein Expression to Protect against Reactive Oxygen Species Damage. PLoS One 10, e0131335
26147969   Curated Info

5

Bunimovich YL, et al. (2014) Deoxycytidine Kinase Augments ATM-Mediated DNA Repair and Contributes to Radiation Resistance. PLoS One 9, e104125
25101980   Curated Info

6

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

7

Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3
23429704   Curated Info

8

Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27
22802335   Curated Info

9

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

10

Assaily W, et al. (2011) ROS-mediated p53 induction of Lpin1 regulates fatty acid oxidation in response to nutritional stress. Mol Cell 44, 491-501
22055193   Curated Info

11

Palmieri D, et al. (2011) HMGA proteins promote ATM expression and enhance cancer cell resistance to genotoxic agents. Oncogene 30, 3024-35
21339738   Curated Info

12

Kozlov SV, et al. (2011) Autophosphorylation and ATM activation: additional sites add to the complexity. J Biol Chem 286, 9107-19
21149446   Curated Info

13

Wang X, et al. (2011) A positive role for c-Abl in Atm and Atr activation in DNA damage response. Cell Death Differ 18, 5-15
20798688   Curated Info

14

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

15

Nam C, Doi K, Nakayama H (2010) Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway. Histol Histopathol 25, 485-93
20183801   Curated Info

16

Li J, Han YR, Plummer MR, Herrup K (2009) Cytoplasmic ATM in neurons modulates synaptic function. Curr Biol 19, 2091-6
19962314   Curated Info

17

Skorta I, et al. (2009) Imatinib mesylate induces cisplatin hypersensitivity in Bcr-Abl+ cells by differential modulation of p53 transcriptional and proapoptotic activity. Cancer Res 69, 9337-45
19934315   Curated Info

18

Daniel JA, et al. (2008) Multiple autophosphorylation sites are dispensable for murine ATM activation in vivo. J Cell Biol 183, 777-83
19047460   Curated Info

19

Wang WH, Hullinger RL, Andrisani OM (2008) Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283, 25455-67
18606816   Curated Info

20

Shields BJ, et al. (2008) DNA replication stalling attenuates tyrosine kinase signaling to suppress S phase progression. Cancer Cell 14, 166-79
18691551   Curated Info

21

Stracker TH, et al. (2008) Chk2 suppresses the oncogenic potential of DNA replication-associated DNA damage. Mol Cell 31, 21-32
18614044   Curated Info

22

Abe Y, et al. (2008) Hedgehog signaling overrides p53-mediated tumor suppression by activating Mdm2. Proc Natl Acad Sci U S A 105, 4838-43
18359851   Curated Info

23

Kremer BE, Adang LA, Macara IG (2007) Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell 130, 837-50
17803907   Curated Info

24

Difilippantonio S, et al. (2007) Distinct domains in Nbs1 regulate irradiation-induced checkpoints and apoptosis. J Exp Med 204, 1003-11
17485521   Curated Info

25

Shi Y, et al. (2007) Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies. J Biol Chem 282, 9236-43
17244605   Curated Info

26

Stiff T, et al. (2006) ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling. EMBO J 25, 5775-82
17124492   Curated Info

27

Pellegrini M, et al. (2006) Autophosphorylation at serine 1987 is dispensable for murine Atm activation in vivo. Nature 443, 222-5
16906133   Curated Info

28

Shreeram S, et al. (2006) Wip1 phosphatase modulates ATM-dependent signaling pathways. Mol Cell 23, 757-64
16949371   Curated Info

29

Ghosh S, et al. (2006) Essential role of tuberous sclerosis genes TSC1 and TSC2 in NF-kappaB activation and cell survival. Cancer Cell 10, 215-26
16959613   Curated Info

30

Zinkel SS, et al. (2005) A role for proapoptotic BID in the DNA-damage response. Cell 122, 579-91
16122425   Curated Info

31

Zhang X, Li J, Sejas DP, Pang Q (2005) The ATM/p53/p21 pathway influences cell fate decision between apoptosis and senescence in reoxygenated hematopoietic progenitor cells. J Biol Chem 280, 19635-40
15753076   Curated Info

32

Hamer G, et al. (2004) Ataxia telangiectasia mutated expression and activation in the testis. Biol Reprod 70, 1206-12
14681204   Curated Info