Ser139
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Home > Phosphorylation Site Page: > Ser139  -  SHC1 (mouse)

Site Information
EEWTRHGsFVNKPtR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448791

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 18 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ) , mutation of modification site ( 18 ) , peptide sequencing ( 18 ) , phosphoamino acid analysis ( 18 )
Disease tissue studied:
melanoma skin cancer ( 16 ) , testicular cancer ( 13 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 , 4 , 7 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 14 ) , 32Dcl3 (myeloid) ( 14 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 18 ) , F9 (testicular) ( 13 ) , fibroblast-lung ( 11 ) , heart ( 9 ) , Hepa 1-6 (epithelial) ( 17 ) , liver ( 1 , 8 , 12 ) , liver [leptin (mouse), homozygous knockout] ( 8 ) , lung ( 9 ) , macrophage-bone marrow ( 10 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 10 ) , RAW 264.7 (macrophage) ( 5 ) , RAW 267.4 (macrophage) ( 15 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 16 ) , spleen ( 9 )

Upstream Regulation
Treatments:
bisindolylmaleimide ( 18 ) , FGF2 ( 18 ) , GSK2334470 ( 4 ) , insulin ( 4 , 7 ) , LPS ( 10 ) , LY294002 ( 4 , 7 ) , MK-2206 ( 4 , 7 ) , PD98059 ( 18 ) , phorbol_ester ( 18 ) , Torin1 ( 4 )

Downstream Regulation
Effects of modification on SHC1:
molecular association, regulation ( 18 )
Induce interaction with:
PTP-PEST (mouse) ( 18 )

References 

1

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

4

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

5

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

8

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

11

Guo A (2010) CST Curation Set: 9810; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

12

Possemato A (2010) CST Curation Set: 9728; Year: 2010; Biosample/Treatment: tissue, liver/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

13

Zhou J (2010) CST Curation Set: 9671; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

14

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

15

Trost M, et al. (2009) The phagosomal proteome in interferon-gamma-activated macrophages. Immunity 30, 143-54
19144319   Curated Info

16

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

17

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

18

Faisal A, el-Shemerly M, Hess D, Nagamine Y (2002) Serine/threonine phosphorylation of ShcA. Regulation of protein-tyrosine phosphatase-pest binding and involvement in insulin signaling. J Biol Chem 277, 30144-52
12052829   Curated Info