Ser674
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Home > Phosphorylation Site Page: > Ser674  -  MLK3 (human)

Site Information
PGRERGEsPTtPPTP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448790

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 1 , 3 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 2 ) , western blotting ( 1 , 2 )
Disease tissue studied:
breast cancer ( 1 , 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
HER2 (human) ( 1 )
Putative in vivo kinases:
Akt1 (human) ( 1 , 3 )
Kinases, in vitro:
Akt1 (human) ( 3 )
Treatments:
anti-HER2 ( 1 ) , lapatinib ( 1 ) , LY294002 ( 1 ) , pictilisib ( 1 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on MLK3:
activity, inhibited ( 1 )
Effects of modification on biological processes:
apoptosis, inhibited ( 1 , 3 ) , carcinogenesis, induced ( 1 )

References 

1

Das S, et al. (2015) Human Epidermal Growth Factor Receptor 2 (HER2) Impedes MLK3 Kinase Activity to Support Breast Cancer Cell Survival. J Biol Chem 290, 21705-12
26152725   Curated Info

2

Rangasamy V, et al. (2010) Estrogen suppresses MLK3-mediated apoptosis sensitivity in ER+ breast cancer cells. Cancer Res 70, 1731-40
20145118   Curated Info

3

Barthwal MK, et al. (2003) Negative regulation of mixed lineage kinase 3 by protein kinase B/AKT leads to cell survival. J Biol Chem 278, 3897-902
12458207   Curated Info