Ser246
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser246  -  FOXO1A (mouse)

Site Information
EGGKSGKsPRRRAAs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 471071

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 5 , 7 ) , mutation of modification site ( 1 , 2 , 6 ) , phospho-antibody ( 1 , 4 , 6 ) , phosphopeptide mapping ( 2 ) , western blotting ( 1 , 2 , 4 , 6 )
Disease tissue studied:
neuroblastoma ( 6 ) , prostate cancer ( 6 )
Relevant cell line - cell type - tissue:
'brain, embryonic' ( 7 ) , 'neuron, cerebellar granule' ( 6 ) , 'neuron, cortical'-brain ( 1 ) , 293T (epithelial) ( 1 , 6 ) , 3T3 (fibroblast) ( 6 ) , 3T3-L1 (fibroblast) ( 5 ) , DU 145 (prostate cell) ( 6 ) , hepatocyte ( 4 ) , hepatocyte-liver ( 2 ) , MEF (fibroblast) ( 6 ) , Neuro-2a (neuron) ( 6 )

Upstream Regulation
Regulatory protein:
SIRT6 (human) ( 4 )
Putative in vivo kinases:
CDK1 (mouse) ( 6 ) , ERK2 (mouse) ( 8 )
Kinases, in vitro:
CDK1 (human) ( 1 ) , CDK2 (human) ( 1 ) , CDK5 (human) ( 1 ) , ERK2 (mouse) ( 8 )
Treatments:
free_fatty_acids ( 4 ) , KCl_withdrawal ( 6 ) , nocodazole ( 6 ) , rosiglitazone ( 4 ) , seliciclib ( 6 )

Downstream Regulation
Effects of modification on FOXO1A:
intracellular localization ( 1 , 2 )
Effects of modification on biological processes:
apoptosis, inhibited ( 1 ) , transcription, induced ( 1 )

References 

1

Zhou J, et al. (2015) The roles of Cdk5-mediated subcellular localization of FOXO1 in neuronal death. J Neurosci 35, 2624-35
25673854   Curated Info

2

Ozcan L, et al. (2012) Calcium signaling through CaMKII regulates hepatic glucose production in fasting and obesity. Cell Metab 15, 739-51
22503562   Curated Info

3

Jiao P, Feng B, Xu H (2012) Mapping MKP-3/FOXO1 Interaction and Evaluating the Effect on Gluconeogenesis. PLoS One 7, e41168
22848439   Curated Info

4

Yang SJ, et al. (2011) Activation of peroxisome proliferator-activated receptor gamma by rosiglitazone increases sirt6 expression and ameliorates hepatic steatosis in rats. PLoS One 6, e17057
21373642   Curated Info

5

Possemato A (2008) CST Curation Set: 4896; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Yuan Z, et al. (2008) Activation of FOXO1 by Cdk1 in cycling cells and postmitotic neurons. Science 319, 1665-8
18356527   Curated Info

7

Possemato A (2008) CST Curation Set: 3828; Year: 2008; Biosample/Treatment: tissue, brain, embryonic/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

8

Asada S, et al. (2007) Mitogen-activated protein kinases, Erk and p38, phosphorylate and regulate Foxo1. Cell Signal 19, 519-27
17113751   Curated Info