Ser249
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Home > Phosphorylation Site Page: > Ser249  -  FOXO1A (human)

Site Information
EGGkSGksPrRrAAs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 471071

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 3 ) , mutation of modification site ( 1 , 3 , 6 , 7 , 8 ) , phospho-antibody ( 1 , 3 , 4 , 6 , 7 , 8 ) , western blotting ( 1 , 3 , 4 , 6 , 7 , 8 )
Disease tissue studied:
liver cancer ( 4 ) , neuroblastoma ( 7 ) , prostate cancer ( 6 , 7 , 8 ) , rhabdomyosarcoma ( 3 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule' ( 7 ) , 'neuron, cortical'-brain ( 1 ) , 293T (epithelial) ( 1 , 7 ) , 3T3 (fibroblast) ( 3 , 7 ) , DU 145 (prostate cell) ( 7 , 8 ) , HepG2 (hepatic) ( 4 ) , LNCaP (prostate cell) ( 6 , 8 ) , MEF (fibroblast) ( 7 ) , Neuro-2a (neuron) ( 7 ) , PC3 (prostate cell) ( 8 ) , Rh30 (muscle cell) ( 3 )

Upstream Regulation
Regulatory protein:
Chk2 (human) ( 8 ) , OSBPL8 (mouse) ( 4 )
Putative in vivo kinases:
CDK1 (human) ( 6 , 7 ) , CDK2 (human) ( 6 , 8 ) , CDK4 (human) ( 3 ) , CDK5 (human) ( 1 )
Kinases, in vitro:
CDK1 (human) ( 6 , 7 ) , CDK2 (mouse) ( 8 ) , CDK4 (human) ( 3 )
Treatments:
camptothecin ( 8 ) , insulin ( 4 ) , ionizing_radiation ( 8 ) , nocodazole ( 7 ) , UCN-01 ( 8 )

Downstream Regulation
Effects of modification on FOXO1A:
activity, inhibited ( 8 ) , intracellular localization ( 2 , 7 , 8 ) , molecular association, regulation ( 7 )
Effects of modification on biological processes:
apoptosis, induced ( 7 ) , apoptosis, inhibited ( 6 , 8 ) , cell cycle regulation ( 6 ) , transcription, altered ( 8 ) , transcription, induced ( 3 , 7 ) , transcription, inhibited ( 6 )
Induce interaction with:
14-3-3 beta (human) ( 7 )

References 

1

Zhou J, et al. (2015) The roles of Cdk5-mediated subcellular localization of FOXO1 in neuronal death. J Neurosci 35, 2624-35
25673854   Curated Info

2

Niedan S, et al. (2014) Suppression of FOXO1 is responsible for a growth regulatory repressive transcriptional sub-signature of EWS-FLI1 in Ewing sarcoma. Oncogene 33, 3927-38
23995784   Curated Info

3

Liu L, Wu J, Ong SS, Chen T (2013) Cyclin-Dependent Kinase 4 Phosphorylates and Positively Regulates PAX3-FOXO1 in Human Alveolar Rhabdomyosarcoma Cells. PLoS One 8, e58193
23469153   Curated Info

4

Jordan SD, et al. (2011) Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nat Cell Biol 13, 434-46
21441927   Curated Info

5

Brent MM, Anand R, Marmorstein R (2008) Structural basis for DNA recognition by FoxO1 and its regulation by posttranslational modification. Structure 16, 1407-16
18786403   Curated Info

6

Liu P, Kao TP, Huang H (2008) CDK1 promotes cell proliferation and survival via phosphorylation and inhibition of FOXO1 transcription factor. Oncogene 27, 4733-44
18408765   Curated Info

7

Yuan Z, et al. (2008) Activation of FOXO1 by Cdk1 in cycling cells and postmitotic neurons. Science 319, 1665-8
18356527   Curated Info

8

Huang H, et al. (2006) CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science 314, 294-7
17038621   Curated Info