Ser67

Site Information
LKSTLsMsPRQRKKM SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447758

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 13 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , mutation of modification site ( 13 ) , phospho-antibody ( 10 , 11 , 12 , 13 ) , western blotting ( 11 , 12 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 3 ) , 'brain, embryonic' ( 8 , 9 ) , 'fat, brown' ( 7 ) , 'neuron, cortical'-brain ( 13 ) , 'neuron, striatal'-brain ( 10 ) , brain ( 5 , 6 , 7 ) , heart ( 4 , 7 , 12 ) , kidney ( 7 ) , lung ( 7 ) , pancreas ( 7 ) , stromal ( 2 ) , testis ( 7 )

Upstream Regulation
Putative in vivo kinases:	CDK5 (mouse) ( 11 , 13 ) , PKCA (mouse) ( 12 )
Kinases, in vitro:	CDK1 (mouse) ( 13 ) , CDK5 (mouse) ( 13 ) , ERK1 (mouse) ( 13 )
Putative upstream phosphatases:	PPP2CA (mouse) ( 11 , 13 ) , PPP3CA (mouse) ( 13 )
Phosphatases, in vitro:	PPP2CA (mouse) ( 13 ) , PPP3CA (mouse) ( 13 )
Treatments:	AMPA ( 11 ) , APV ( 11 ) , calyculin_A ( 11 ) , ciclosporin ( 13 ) , depolarization ( 11 ) , EGTA ( 11 ) , kainic_acid ( 11 ) , MK801 ( 11 ) , Ni(2+) ( 11 ) , nimodipine ( 11 ) , NMDA ( 11 , 13 ) , okadaic_acid ( 11 , 13 ) , phorbol_ester ( 10 ) , Ro-32-0432 ( 10 ) , seliciclib ( 13 )

References
1	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
2	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
3	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
4	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
5	Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29 22645316 Curated Info
6	Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9 22807455 Curated Info
7	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
8	Zhou J (2009) CST Curation Set: 7428; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSP Curated Info
9	Zhou J (2009) CST Curation Set: 7429; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSP Curated Info
10	Sahin B, et al. (2008) Negative regulation of cyclin-dependent kinase 5 targets by protein kinase C. Eur J Pharmacol 581, 270-5 18190909 Curated Info
11	Nguyen C, et al. (2007) Differential regulation of the Cdk5-dependent phosphorylation sites of inhibitor-1 and DARPP-32 by depolarization. J Neurochem 103, 1582-93 17868322 Curated Info
12	Braz JC, et al. (2004) PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nat Med 10, 248-54 14966518 Curated Info
13	Bibb JA, et al. (2001) Phosphorylation of protein phosphatase inhibitor-1 by Cdk5. J Biol Chem 276, 14490-7 11278334 Curated Info