Ser729
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser729  -  PKCE (mouse)

Site Information
QEEFKGFsyFGEDLM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448789
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 18 ) , immunoprecipitation ( 11 ) , mass spectrometry ( 2 , 4 , 6 , 7 , 8 , 9 , 10 , 13 , 14 , 15 , 16 , 19 ) , mutation of modification site ( 17 ) , phospho-antibody ( 11 , 12 , 17 , 19 ) , western blotting ( 11 , 12 , 17 )
Relevant cell line - cell type - tissue:
'brain, embryonic' ( 15 ) , 293 (epithelial) ( 17 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 19 ) , 3T3 (fibroblast) ( 18 ) , 3T6 (fibroblast) ( 18 , 19 ) , brain ( 7 , 8 , 9 , 10 , 14 , 16 ) , C2C12 (myoblast) ( 11 ) , embryo ( 8 ) , heart ( 6 ) , hepatocyte-liver ( 12 ) , liver ( 4 , 8 , 12 ) , neuron:postsynaptic density-'brain, hippocampus, CA1 region' ( 2 )

Upstream Regulation
Regulatory protein:
LPIN1 (mouse) ( 12 ) , PKCD (mouse) ( 18 ) , TORC2 (mouse) ( 12 )
Phosphatases, in vitro:
PPP2CB (human) ( 17 )
Treatments:
ascomycin ( 18 ) , calyculin_A ( 18 ) , cell_adhesion ( 18 ) , chelerythrine ( 18 ) , ciclosporin ( 18 ) , collagen_IV ( 17 ) , cytochalasin_D ( 18 ) , fibronectin ( 17 ) , high_cell_density ( 18 ) , insulin ( 11 , 12 ) , LLnL ( 18 ) , LY294002 ( 18 ) , nocodazole ( 18 ) , okadaic_acid ( 18 ) , PA ( 12 ) , PD98059 ( 18 ) , peptide inhibitor ( 18 ) , phorbol_ester ( 18 ) , rapamycin ( 18 ) , Ro31-8220 ( 18 ) , serum ( 18 ) , serum_starvation ( 18 ) , siRNA ( 18 ) , wortmannin ( 18 )

Downstream Regulation
Effects of modification on PKCE:
enzymatic activity, induced ( 17 ) , intracellular localization ( 11 , 19 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Li J, et al. (2016) Long-term potentiation modulates synaptic phosphorylation networks and reshapes the structure of the postsynaptic interactome. Sci Signal 9, rs8
27507650   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

7

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

8

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

11

Gaboardi GC, et al. (2010) A role for PKCepsilon during C2C12 myogenic differentiation. Cell Signal 22, 629-35
19954762   Curated Info

12

Ryu D, et al. (2009) TORC2 regulates hepatic insulin signaling via a mammalian phosphatidic acid phosphatase, LIPIN1. Cell Metab 9, 240-51
19254569   Curated Info

13

Guo A (2007) CST Curation Set: 3504; Year: 2007; Biosample/Treatment: cell line, E6-rictor+/+/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS*/T*F/Y) Immunoaffinity Beads Cat#: 1992
Curated Info

14

Guo A (2007) CST Curation Set: 3121; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS*/T*F/Y) Immunoaffinity Beads Cat#: 1992
Curated Info

15

Guo A (2007) CST Curation Set: 2717; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST](D/E)X(D/E)
Curated Info

16

Guo A (2007) CST Curation Set: 2718; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST](D/E)X(D/E)
Curated Info

17

Ivaska J, Bosca L, Parker PJ (2003) PKCepsilon is a permissive link in integrin-dependent IFN-gamma signalling that facilitates JAK phosphorylation of STAT1. Nat Cell Biol 5, 363-9
12640464   Curated Info

18

England K, et al. (2001) Signalling pathways regulating the dephosphorylation of Ser729 in the hydrophobic domain of protein kinase Cepsilon upon cell passage. J Biol Chem 276, 10437-42
11121415   Curated Info

19

England K, Rumsby MG (2000) Changes in protein kinase C epsilon phosphorylation status and intracellular localization as 3T3 and 3T6 fibroblasts grow to confluency and quiescence: a role for phosphorylation at ser-729? Biochem J 352 Pt 1, 19-26
11062054   Curated Info