Ser729
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Home > Phosphorylation Site Page: > Ser729  -  PKCE (human)

Site Information
QEEFKGFsYFGEDLM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448789

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 14 ) , electrophoretic mobility shift ( 16 ) , immunoprecipitation ( 14 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 10 , 11 , 12 , 13 ) , mutation of modification site ( 16 , 18 ) , phospho-antibody ( 9 , 14 , 15 , 16 ) , phosphoamino acid analysis ( 17 ) , western blotting ( 9 , 14 , 15 , 16 , 17 , 18 )
Disease tissue studied:
bladder cancer ( 17 ) , breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 , 3 ) , lung cancer ( 12 , 14 ) , non-small cell lung cancer ( 12 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 6 ) , 293 (epithelial) ( 9 , 16 , 17 ) , 3T3 (fibroblast) ( 16 ) , A498 (renal) ( 11 ) , A549 (pulmonary) ( 14 ) , brain ( 18 ) , breast ( 1 , 3 ) , cardiac ( 15 ) , COS (fibroblast) ( 18 ) , HeLa (cervical) ( 2 ) , HeLa S3 (cervical) ( 13 ) , HeLa_Meta (cervical) ( 10 ) , HeLa_Pro (cervical) ( 10 ) , HeLa_Telo (cervical) ( 10 ) , lung ( 12 ) , UMUC3 (bladder cell) ( 17 )

Upstream Regulation
Putative in vivo kinases:
PKCE (human) ( 16 )
Kinases, in vitro:
PKCE (human) ( 14 , 16 )
Phosphatases, in vitro:
PPP2CA (human) ( 16 )
Treatments:
angiotensin_2 ( 15 ) , bisindolylmaleimide ( 16 ) , etoposide ( 5 ) , ionizing_radiation ( 14 ) , MG132_withdrawal ( 10 ) , p-Tyr ( 14 ) , PDGF ( 16 ) , PKC-epsilon_translocation_inhibitor ( 5 ) , siRNA ( 14 )

Downstream Regulation
Effects of modification on PKCE:
enzymatic activity, induced ( 16 , 18 ) , intracellular localization ( 18 ) , molecular association, regulation ( 18 )
Inhibit interaction with:
AKAP9 (human) ( 18 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

5

Lau E, et al. (2012) PKCĪµ promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Cell 148, 543-55
22304920   Curated Info

6

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

7

Rikova K (2011) CST Curation Set: 13128; Year: 2011; Biosample/Treatment: cell line, MGH-6/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

8

Rikova K (2011) CST Curation Set: 13131; Year: 2011; Biosample/Treatment: cell line, MGH-9/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

9

Cameron AJ, et al. (2011) mTORC2 targets AGC kinases through Sin1-dependent recruitment. Biochem J 439, 287-97
21806543   Curated Info

10

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

11

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

12

Rikova K (2010) CST Curation Set: 9452; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

13

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

14

Wanner G, et al. (2008) Activation of protein kinase Cepsilon stimulates DNA-repair via epidermal growth factor receptor nuclear accumulation. Radiother Oncol 86, 383-90
18037521   Curated Info

15

Stawowy P, et al. (2005) Protein kinase C epsilon mediates angiotensin II-induced activation of beta1-integrins in cardiac fibroblasts. Cardiovasc Res 67, 50-9
15949469   Curated Info

16

Cenni V, et al. (2002) Regulation of novel protein kinase C epsilon by phosphorylation. Biochem J 363, 537-45
11964154   Curated Info

17

Parekh DB, et al. (2000) Beta1-integrin and PTEN control the phosphorylation of protein kinase C. Biochem J 352 Pt 2, 425-33
11085936   Curated Info

18

Takahashi M, et al. (2000) Association of immature hypophosphorylated protein kinase cepsilon with an anchoring protein CG-NAP. J Biol Chem 275, 34592-6
10945988   Curated Info