Thr370
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Home > Phosphorylation Site Page: > Thr370  -  MOR-1 (mouse)

Site Information
sARIRQNtREHPstA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447785

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , mass spectrometry ( 3 , 5 , 10 ) , mutation of modification site ( 2 , 4 , 9 , 10 , 11 ) , phospho-antibody ( 2 , 5 , 6 , 9 , 11 ) , western blotting ( 2 , 5 , 6 , 9 , 11 )
Disease tissue studied:
pituitary cancer ( 11 )
Relevant cell line - cell type - tissue:
'brain, striatum' ( 11 ) , 293 (epithelial) ( 2 , 6 , 9 , 10 , 11 ) , AtT20 (pituitary cell) ( 11 ) , brain ( 3 , 5 ) , neuron-brain ( 4 )

Upstream Regulation
Regulatory protein:
PKCA (human) ( 9 ) , PKCA (mouse) ( 6 ) , PKCB (mouse) ( 6 ) , PKCB iso2 (mouse) ( 6 ) , PKCE (mouse) ( 6 ) , PKCG (mouse) ( 6 )
Putative in vivo kinases:
GRK2 (human) ( 2 , 8 ) , GRK3 (human) ( 2 , 8 ) , PKCA (human) ( 6 )
Treatments:
BIM23627 ( 6 ) , ConA ( 9 ) , DAMGO ( 2 , 6 , 8 , 9 ) , etonitazene ( 3 , 5 , 9 ) , fentanyl ( 3 , 5 , 11 ) , GRK2_inhibitor ( 6 ) , H-89 ( 6 ) , LY333531 ( 6 ) , morphine ( 2 , 3 , 5 , 6 , 10 ) , mutation ( 5 ) , naloxone ( 9 , 11 ) , painful stimulus ( 11 ) , phorbol_ester ( 6 , 9 ) , Ro-32-0432 ( 6 ) , siRNA ( 2 )

Downstream Regulation
Effects of modification on MOR-1:
molecular association, regulation ( 2 ) , receptor internalization, induced ( 8 ) , receptor recycling, induced ( 4 )
Induce interaction with:
ARRB2 (human) ( 2 )

References 

1

Gillis A, et al. (2020) Low intrinsic efficacy for G protein activation can explain the improved side effect profiles of new opioid agonists. Sci Signal 13
32234959   Curated Info

2

Miess E, et al. (2018) Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal 11
30018083   Curated Info

3

Moulédous L, et al. (2015) Phosphoproteomic analysis of the mouse brain mu-opioid (MOP) receptor. FEBS Lett 589, 2401-8
26226422   Curated Info

4

Bowman SL, et al. (2015) Cell-autonomous regulation of Mu-opioid receptor recycling by substance P. Cell Rep 10, 1925-36
25801029   Curated Info

5

Glück L, et al. (2014) Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry 76, 767-74
24629717   Curated Info

6

Illing S, Mann A, Schulz S (2014) Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C. Br J Pharmacol 171, 1330-40
24308893   Curated Info

7

Just S, et al. (2013) Differentiation of opioid drug effects by hierarchical multi-site phosphorylation. Mol Pharmacol 83, 633-9
23239825   Curated Info

8

Doll C, et al. (2012) Deciphering µ-opioid receptor phosphorylation and dephosphorylation in HEK293 cells. Br J Pharmacol 167, 1259-70
22725608   Curated Info

9

Doll C, et al. (2011) Agonist-selective patterns of µ-opioid receptor phosphorylation revealed by phosphosite-specific antibodies. Br J Pharmacol 164, 298-307
21449911   Curated Info

10

Lau EK, et al. (2011) Quantitative encoding of the effect of a partial agonist on individual opioid receptors by multisite phosphorylation and threshold detection. Sci Signal 4, ra52
21868358   Curated Info

11

Petraschka M, et al. (2007) The absence of endogenous beta-endorphin selectively blocks phosphorylation and desensitization of mu opioid receptors following partial sciatic nerve ligation. Neuroscience 146, 1795-807
17467916   Curated Info