Ser727
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Home > Phosphorylation Site Page: > Ser727  -  STAT1 (rat)

Site Information
TENLLPMsPEEFDEM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447753
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 4 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 6 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 ) , western blotting ( 3 , 5 , 6 , 7 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 1 ) , 'brain, cerebellum' ( 1 ) , 'brain, cerebral cortex' ( 5 ) , adipose tissue ( 1 ) , brain ( 2 ) , FRTL-5 (thyroid cell) ( 7 ) , heart ( 1 , 3 , 6 ) , hepatocyte-liver ( 4 ) , liver ( 1 ) , lung ( 1 ) , myocyte-heart ( 6 ) , spleen ( 1 ) , stomach ( 1 ) , thymus ( 1 ) , U3A (fibroblast) ( 6 )

Upstream Regulation
Treatments:
colforsin ( 7 ) , fludarabine ( 4 ) , ganglioside ( 5 ) , hypoxia/reoxygenation ( 4 ) , IFN-gamma ( 7 ) , IL-6 ( 4 ) , ischemia ( 3 , 6 ) , ischemia/reperfusion ( 6 ) , NAC ( 4 ) , SB203580 ( 6 ) , TSH ( 7 ) , wortmannin ( 4 )

Downstream Regulation
Effects of modification on STAT1:
activity, induced ( 4 )
Effects of modification on biological processes:
apoptosis, induced ( 6 ) , apoptosis, inhibited ( 4 ) , transcription, altered ( 4 , 6 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Guo A (2007) CST Curation Set: 2243; Year: 2007; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

3

McCormick J, et al. (2006) Free radical scavenging inhibits STAT phosphorylation following in vivo ischemia/reperfusion injury. FASEB J 20, 2115-7
16935931   Curated Info

4

Terui K, et al. (2004) Hypoxia/re-oxygenation-induced, redox-dependent activation of STAT1 (signal transducer and activator of transcription 1) confers resistance to apoptotic cell death via hsp70 induction. Biochem J 380, 203-9
14984365   Curated Info

5

Kim OS, Park EJ, Joe EH, Jou I (2002) JAK-STAT signaling mediates gangliosides-induced inflammatory responses in brain microglial cells. J Biol Chem 277, 40594-601
12191995   Curated Info

6

Stephanou A, et al. (2001) Induction of apoptosis and Fas receptor/Fas ligand expression by ischemia/reperfusion in cardiac myocytes requires serine 727 of the STAT-1 transcription factor but not tyrosine 701. J Biol Chem 276, 28340-7
11309387   Curated Info

7

Park ES, et al. (2000) Thyrotropin induces SOCS-1 (suppressor of cytokine signaling-1) and SOCS-3 in FRTL-5 thyroid cells. Mol Endocrinol 14, 440-8
10707961   Curated Info