Ser643

Site Information
LNEKPQLsFsDKNLI SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448591

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 10 ) , mutation of modification site ( 10 ) , phospho-antibody ( 1 , 5 , 6 , 7 , 8 , 9 , 10 ) , western blotting ( 5 , 6 , 10 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'muscle, skeletal' ( 9 ) , adipose tissue ( 4 ) , COS (fibroblast) ( 10 ) , heart ( 4 , 6 ) , IEC-6 (epithelial) ( 2 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 ) , lung ( 4 ) , myocyte-heart ( 1 , 7 , 8 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 3 ) , PC-12 (chromaffin) ( 3 ) , RIE (epithelial) ( 5 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 ) , thymus ( 4 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 , 10 ) , mutation of modification site ( 10 ) , phospho-antibody ( 1 , 5 , 6 , 7 , 8 , 9 , 10 ) , western blotting ( 5 , 6 , 10 )

Relevant cell line - cell type - tissue:

'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'muscle, skeletal' ( 9 ) , adipose tissue ( 4 ) , COS (fibroblast) ( 10 ) , heart ( 4 , 6 ) , IEC-6 (epithelial) ( 2 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 ) , lung ( 4 ) , myocyte-heart ( 1 , 7 , 8 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 3 ) , PC-12 (chromaffin) ( 3 ) , RIE (epithelial) ( 5 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 ) , thymus ( 4 )

Upstream Regulation
Regulatory protein:	ITGA5 (human) ( 5 )
Treatments:	cytochalasin_D ( 5 ) , endothelin ( 8 ) , fibronectin ( 5 ) , GF109203X ( 8 ) , H-CHO ( 9 ) , H2O2 ( 10 ) , insulin ( 9 ) , KBR ( 7 ) , leucine ( 9 ) , LY294002 ( 5 , 8 ) , meal feeding ( 6 , 9 ) , norleucine ( 9 ) , OXO ( 5 ) , phorbol_ester ( 8 ) , PP2 ( 5 , 8 ) , serum ( 2 ) , sevoflurane ( 7 )

Downstream Regulation
Effects of modification on PKCD:	enzymatic activity, induced ( 8 )

References
1	Gong J, Holewinski RJ, Van Eyk JE, Steinberg SF (2016) A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain contributes to the activation of protein kinase C-δ. Biochem J 473, 311-20 26546672 Curated Info
2	Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669 23712012 Curated Info
3	Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30 22027198 Curated Info
4	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
5	Oh MA, et al. (2007) PKCdelta and cofilin activation affects peripheral actin reorganization and cell-cell contact in cells expressing integrin alpha5 but not its tailless mutant. J Cell Sci 120, 2717-30 17646675 Curated Info
6	Vary TC, Lynch CJ (2006) Meal feeding stimulates phosphorylation of multiple effector proteins regulating protein synthetic processes in rat hearts. J Nutr 136, 2284-90 16920842 Curated Info
7	Bouwman RA, et al. (2006) Cardioprotection via activation of protein kinase C-delta depends on modulation of the reverse mode of the Na+/Ca2+ exchanger. Circulation 114, I226-32 16820577 Curated Info
8	Markou T, Yong CS, Sugden PH, Clerk A (2006) Regulation of protein kinase C delta by phorbol ester, endothelin-1, and platelet-derived growth factor in cardiac myocytes. J Biol Chem 281, 8321-31 16361709 Curated Info
9	Vary TC, Goodman S, Kilpatrick LE, Lynch CJ (2005) Nutrient regulation of PKCepsilon is mediated by leucine, not insulin, in skeletal muscle. Am J Physiol Endocrinol Metab 289, E684-94 15886222 Curated Info
10	Konishi H, et al. (2001) Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro. Proc Natl Acad Sci U S A 98, 6587-92 11381116 Curated Info