Ser19
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Home > Phosphorylation Site Page: > Ser19  -  CRYAB (mouse)

Site Information
RPFFPFHsPSRLFDQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447728

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 4 , 5 , 6 ) , mutation of modification site ( 1 , 7 ) , phospho-antibody ( 1 , 8 ) , western blotting ( 1 , 8 )
Disease tissue studied:
brain cancer ( 5 ) , neuroendocrine cancer ( 5 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [KRas (human), transfection] ( 3 ) , brain ( 5 , 6 ) , heart ( 4 ) , HL-1 (myocyte) ( 1 ) , myocyte-heart ( 7 , 8 )

Upstream Regulation
Regulatory protein:
KRas (mouse) ( 3 ) , MKK6 (human) ( 8 )
Treatments:
SB203580 ( 8 ) , virus infection ( 1 )

Downstream Regulation
Effects of modification on CRYAB:
protein degradation ( 1 )

References 

1

Fung G, et al. (2017) Phosphorylation and degradation of αB-crystallin during enterovirus infection facilitates viral replication and induces viral pathogenesis. Oncotarget 8, 74767-74780
29088822   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80
23608596   Curated Info

4

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

5

Guo A (2011) CST Curation Set: 12734; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: brain cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

6

Guo A (2008) CST Curation Set: 5005; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

7

Jin JK, et al. (2008) Localization of phosphorylated {alpha}B-crystallin to heart mitochondria during ischemia-reperfusion. Am J Physiol Heart Circ Physiol 294, H337-44
17993600   Curated Info

8

Hoover HE, Thuerauf DJ, Martindale JJ, Glembotski CC (2000) alpha B-crystallin gene induction and phosphorylation by MKK6-activated p38. A potential role for alpha B-crystallin as a target of the p38 branch of the cardiac stress response. J Biol Chem 275, 23825-33
10816593   Curated Info

9

Kato K, et al. (1998) Phosphorylation of alphaB-crystallin in mitotic cells and identification of enzymatic activities responsible for phosphorylation. J Biol Chem 273, 28346-54
9774459   Curated Info