Ser45
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Home > Phosphorylation Site Page: > Ser45  -  CRYAB (mouse)

Site Information
FSTATSLsPFYLRPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448748

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , mass spectrometry ( 3 ) , mutation of modification site ( 1 , 5 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 1 , 4 , 5 , 7 , 8 )
Disease tissue studied:
brain cancer ( 3 ) , neuroendocrine cancer ( 3 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 8 ) , astrocyte ( 2 ) , brain ( 2 , 3 ) , C2C12 (myoblast) ( 6 ) , heart ( 5 ) , heart [PKN1 (mouse), transgenic] ( 4 ) , HL-1 (myocyte) ( 1 ) , myocyte-heart ( 5 , 7 ) , myocyte-heart [PKN1 (mouse), transgenic] ( 4 ) , Swiss 3T3 (fibroblast) ( 8 )

Upstream Regulation
Regulatory protein:
MKK6 (human) ( 7 ) , PKN1 (mouse) ( 4 )
Putative in vivo kinases:
ERK1 (human) ( 2 ) , PKN1 (mouse) ( 4 )
Treatments:
arsenite ( 7 ) , heat_shock ( 6 ) , ischemia/reperfusion ( 5 ) , LPS ( 2 ) , oxidative_stress ( 2 ) , PD98059 ( 2 , 7 ) , SB203580 ( 7 ) , sorbitol ( 7 ) , virus infection ( 1 )

Downstream Regulation
Effects of modification on CRYAB:
intracellular localization ( 2 ) , molecular association, regulation ( 2 ) , protein degradation ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 )

References 

1

Fung G, et al. (2017) Phosphorylation and degradation of αB-crystallin during enterovirus infection facilitates viral replication and induces viral pathogenesis. Oncotarget 8, 74767-74780
29088822   Curated Info

2

Kuipers HF, et al. (2017) Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114, E1745-E1754
28196893   Curated Info

3

Guo A (2011) CST Curation Set: 12734; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: brain cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

4

Takagi H, et al. (2010) Activation of PKN mediates survival of cardiac myocytes in the heart during ischemia/reperfusion. Circ Res 107, 642-9
20595653   Curated Info

5

Jin JK, et al. (2008) Localization of phosphorylated {alpha}B-crystallin to heart mitochondria during ischemia-reperfusion. Am J Physiol Heart Circ Physiol 294, H337-44
17993600   Curated Info

6

Launay N, et al. (2006) Cell signaling pathways to alphaB-crystallin following stresses of the cytoskeleton. Exp Cell Res 312, 3570-84
16979163   Curated Info

7

Hoover HE, Thuerauf DJ, Martindale JJ, Glembotski CC (2000) alpha B-crystallin gene induction and phosphorylation by MKK6-activated p38. A potential role for alpha B-crystallin as a target of the p38 branch of the cardiac stress response. J Biol Chem 275, 23825-33
10816593   Curated Info

8

Kato K, et al. (1998) Phosphorylation of alphaB-crystallin in mitotic cells and identification of enzymatic activities responsible for phosphorylation. J Biol Chem 273, 28346-54
9774459   Curated Info