Thr197
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Home > Phosphorylation Site Page: > Thr197  -  p27Kip1 (mouse)

Site Information
KPGLRRQt_______   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448781

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 5 ) , mutation of modification site ( 3 , 4 , 6 ) , phospho-antibody ( 2 , 3 , 6 ) , western blotting ( 2 , 3 , 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
LKB1 (mouse) ( 6 )
Putative in vivo kinases:
CAMK1A (human) ( 1 )
Kinases, in vitro:
AMPKA1 (human) ( 6 ) , AMPKA1 (rat) ( 3 , 4 )
Treatments:
acadesine ( 6 ) , glucose_starvation ( 3 , 6 ) , IL-3 ( 2 ) , ponasterone_A ( 2 ) , siRNA ( 6 )

References 

1

Mallampalli RK, et al. (2013) Fbxl12 triggers G1 arrest by mediating degradation of calmodulin kinase I. Cell Signal 25, 2047-59
23707388   Curated Info

2

Bustany S, Tchakarska G, Sola B (2011) Cyclin D1 regulates p27(Kip1) stability in B cells. Cell Signal 23, 171-9
20837141   Curated Info

3

Short JD, et al. (2010) AMPK-mediated phosphorylation of murine p27 at T197 promotes binding of 14-3-3 proteins and increases p27 stability. Mol Carcinog 49, 429-39
20146253   Curated Info

4

Short JD, et al. (2008) AMP-activated protein kinase signaling results in cytoplasmic sequestration of p27. Cancer Res 68, 6496-506
18701472   Curated Info

5

Possemato A (2008) CST Curation Set: 3848; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

6

Liang J, et al. (2007) The energy sensing LKB1-AMPK pathway regulates p27(kip1) phosphorylation mediating the decision to enter autophagy or apoptosis. Nat Cell Biol 9, 218-24
17237771   Curated Info