Ser92
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Home > Phosphorylation Site Page: > Ser92  -  BRF1 (human)

Site Information
RFRDRsFsEGGERLL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470147

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 11 ) , mutation of modification site ( 3 , 12 , 13 , 14 ) , phospho-antibody ( 3 , 13 , 14 ) , western blotting ( 3 , 14 )
Disease tissue studied:
breast cancer ( 6 ) , breast ductal carcinoma ( 6 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 6 ) , fibrosarcoma ( 12 ) , lung cancer ( 7 ) , non-small cell lung adenocarcinoma ( 7 ) , melanoma skin cancer ( 4 ) , fibrosarcoma of soft tissue ( 12 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Akt1 (human) ( 14 ) , MAPKAPK2 (human) ( 12 )
Kinases, in vitro:
Akt1 (human) ( 14 ) , MAPKAPK2 (human) ( 12 )
Treatments:
anisomycin ( 12 ) , insulin ( 14 ) , ischemia ( 6 ) , vemurafenib ( 4 ) , wortmannin ( 14 )

Downstream Regulation
Effects of modification on BRF1:
intracellular localization ( 13 ) , molecular association, regulation ( 12 , 13 , 14 ) , protein stabilization ( 13 )
Effects of modification on biological processes:
cell growth, inhibited ( 3 ) , RNA stability, altered ( 13 ) , transcription, altered ( 12 )
Induce interaction with:
14-3-3 beta (human) ( 12 , 13 , 14 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell
32645325   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Herranz N, et al. (2015) mTOR regulates MAPKAPK2 translation to control the senescence-associated secretory phenotype. Nat Cell Biol 17, 1205-17
26280535   Curated Info

4

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

8

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

11

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

12

Maitra S, et al. (2008) The AU-rich element mRNA decay-promoting activity of BRF1 is regulated by mitogen-activated protein kinase-activated protein kinase 2. RNA 14, 950-9
18326031   Curated Info

13

Benjamin D, et al. (2006) BRF1 protein turnover and mRNA decay activity are regulated by protein kinase B at the same phosphorylation sites. Mol Cell Biol 26, 9497-507
17030608   Curated Info

14

Schmidlin M, et al. (2004) The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase B. EMBO J 23, 4760-9
15538381   Curated Info