Ser731
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Home > Phosphorylation Site Page: > Ser731  -  STAT5B (human)

Site Information
TyMDQAPsPAVCPQA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448751

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 5 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 ) , western blotting ( 1 , 3 , 4 , 6 , 7 )
Disease tissue studied:
breast cancer ( 5 ) , leukemia ( 6 , 7 ) , acute myelogenous leukemia ( 6 , 7 ) , lymphoma ( 6 ) , Burkitt's lymphoma ( 6 )
Relevant cell line - cell type - tissue:
BaF3 ('B lymphocyte, precursor') ( 7 ) , Daudi (B lymphocyte) ( 6 ) , KG-1 (myeloid) ( 6 ) , MEF (fibroblast) ( 6 ) , SET2 ( 1 ) , SKBr3 (breast cell) ( 5 ) , UT-7 (myeloid) ( 7 ) , YT ( 3 , 4 )

Upstream Regulation
Regulatory protein:
CIP2A (human) ( 1 )
Putative upstream phosphatases:
PPP2CA (human) ( 1 )
Treatments:
AG490 ( 8 ) , calyculin_A ( 1 , 4 ) , ciclosporin ( 4 ) , dactolisib ( 1 ) , EGF ( 5 ) , Epo ( 7 ) , genistein ( 6 ) , IFN-alpha ( 6 ) , IFN-beta ( 6 ) , IL-2 ( 3 , 4 , 8 ) , IL-3 ( 7 ) , LY294002 ( 6 ) , NVP-BKM120 ( 1 ) , okadaic_acid ( 4 ) , PD98059 ( 6 ) , RAD001 ( 1 ) , SB203580 ( 6 ) , SGI-1776 ( 2 ) , tautomycin ( 4 )

Downstream Regulation
Effects of modification on STAT5B:
activity, induced ( 5 )
Effects of modification on biological processes:
transcription, altered ( 5 )

References 

1

Bartalucci N, et al. (2017) Inhibitors of the PI3K/mTOR pathway prevent STAT5 phosphorylation in JAK2V617F mutated cells through PP2A/CIP2A axis. Oncotarget 8, 96710-96724
29228564   Curated Info

2

Guo Z, et al. (2014) PIM inhibitors target CD25-positive AML cells through concomitant suppression of STAT5 activation and degradation of MYC oncogene. Blood 124, 1777-89
25006129   Curated Info

3

Mitra A, et al. (2012) Signal transducer and activator of transcription 5b (Stat5b) serine 193 is a novel cytokine-induced phospho-regulatory site that is constitutively activated in primary hematopoietic malignancies. J Biol Chem 287, 16596-608
22442148   Curated Info

4

Ross JA, et al. (2010) Protein Phosphatase 2A Regulates Interleukin-2 Receptor Complex Formation and JAK3/STAT5 Activation. J Biol Chem 285, 3582-91
19923221   Curated Info

5

Weaver AM, Silva CM (2007) S731 in the transactivation domain modulates STAT5b activity. Biochem Biophys Res Commun 362, 1026-30
17822672   Curated Info

6

Uddin S, et al. (2003) Role of Stat5 in type I interferon-signaling and transcriptional regulation. Biochem Biophys Res Commun 308, 325-30
12901872   Curated Info

7

Haq R, et al. (2002) Regulation of erythropoietin-induced STAT serine phosphorylation by distinct mitogen-activated protein kinases. J Biol Chem 277, 17359-66
11875080   Curated Info

8

Kirken RA, et al. (2000) Functional uncoupling of the Janus kinase 3-Stat5 pathway in malignant growth of human T cell leukemia virus type 1-transformed human T cells. J Immunol 165, 5097-104
11046040   Curated Info