Ser45
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Home > Phosphorylation Site Page: > Ser45  -  CRYAB (rat)

Site Information
FSTATSLsPFYLRPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448748

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 8 ) , mutation of modification site ( 1 , 9 , 10 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 7 , 8 , 11 , 12 , 13 ) , western blotting ( 2 , 3 , 4 , 5 , 7 , 8 , 11 , 12 , 13 )
Disease tissue studied:
diabetes mellitus ( 7 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 11 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 1 ) , chondrocyte ( 3 ) , heart ( 4 , 12 , 13 ) , myocyte-heart ( 9 , 10 ) , neuron-'brain, hippocampus' ( 1 ) , retina ( 8 ) , retinal-retina ( 2 , 5 , 7 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (human) ( 13 ) , ERK2 (human) ( 13 )
Treatments:
anisomycin ( 13 ) , bisindolylmaleimide ( 12 , 13 ) , diabetes ( 4 , 5 ) , DRB ( 3 ) , genistein ( 12 , 13 ) , H2O2 ( 12 ) , injury ( 2 ) , ischemia ( 13 ) , ischemia/reperfusion ( 8 ) , muscle atrophy ( 11 ) , nerve_damage ( 11 ) , PD98059 ( 12 , 13 ) , phenylephrine ( 13 ) , phorbol_ester ( 13 ) , SB203580 ( 12 , 13 ) , staurosporine ( 12 ) , TBB ( 3 ) , triciribine ( 3 )

Downstream Regulation
Effects of modification on CRYAB:
intracellular localization ( 5 ) , phosphorylation ( 8 ) , protein degradation ( 1 )
Effects of modification on biological processes:
apoptosis, altered ( 10 ) , cytoskeletal reorganization ( 1 ) , signaling pathway regulation ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
diabetes mellitus ( 7 )

References 

1

Bartelt-Kirbach B, et al. (2020) HspB5/αB-crystallin phosphorylation at S45 and S59 is essential for protection of the dendritic tree of rat hippocampal neurons. J Neurochem
33220080   Curated Info

2

Schmidt T, et al. (2016) Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/αB-crystallin in the rat retina upon optic nerve injury. Cell Stress Chaperones 21, 167-78
26475352   Curated Info

3

Lee SW, et al. (2016) Alpha B-Crystallin Protects Rat Articular Chondrocytes against Casein Kinase II Inhibition-Induced Apoptosis. PLoS One 11, e0166450
27851782   Curated Info

4

Reddy VS, Kumar ChU, Raghu G, Reddy GB (2014) Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions. Arch Biochem Biophys 558, 1-9
24950024   Curated Info

5

Reddy VS, et al. (2013) Response of small heat shock proteins in diabetic rat retina. Invest Ophthalmol Vis Sci 54, 7674-82
24159092   Curated Info

6

Krishnamoorthy V, Donofrio AJ, Martin JL (2013) O-GlcNAcylation of αB-crystallin regulates its stress-induced translocation and cytoprotection. Mol Cell Biochem 379, 59-68
23543138   Curated Info

7

Heise EA, et al. (2013) Strain-independent increases of crystallin proteins in the retina of type 1 diabetic rats. PLoS One 8, e82520
24349305   Curated Info

8

Hong SM, Yang YS (2012) A Potential Role of Crystallin in the Vitreous Bodies of Rats after Ischemia-reperfusion Injury. Korean J Ophthalmol 26, 248-54
22870022   Curated Info

9

Jin JK, et al. (2008) Localization of phosphorylated {alpha}B-crystallin to heart mitochondria during ischemia-reperfusion. Am J Physiol Heart Circ Physiol 294, H337-44
17993600   Curated Info

10

Morrison LE, Hoover HE, Thuerauf DJ, Glembotski CC (2003) Mimicking phosphorylation of alphaB-crystallin on serine-59 is necessary and sufficient to provide maximal protection of cardiac myocytes from apoptosis. Circ Res 92, 203-11
12574148   Curated Info

11

Kato K, et al. (2002) Innervation-dependent phosphorylation and accumulation of alphaB-crystallin and Hsp27 as insoluble complexes in disused muscle. FASEB J 16, 1432-4
12205038   Curated Info

12

Eaton P, Fuller W, Shattock MJ (2002) S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation. J Biol Chem 277, 21189-96
11925435   Curated Info

13

Eaton P, Fuller W, Bell JR, Shattock MJ (2001) AlphaB crystallin translocation and phosphorylation: signal transduction pathways and preconditioning in the isolated rat heart. J Mol Cell Cardiol 33, 1659-71
11549345   Curated Info

14

Kato K, et al. (1998) Phosphorylation of alphaB-crystallin in mitotic cells and identification of enzymatic activities responsible for phosphorylation. J Biol Chem 273, 28346-54
9774459   Curated Info