Ser65
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Home > Phosphorylation Site Page: > Ser65  -  MAFA (mouse)

Site Information
PCSsVPSsPSFCAPS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 5403102

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mutation of modification site ( 1 , 2 , 4 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 2 , 4 )
Disease tissue studied:
pancreatic cancer ( 1 , 4 ) , pancreatic carcinoma ( 1 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
PSME3 (human) ( 2 )
Putative in vivo kinases:
GSK3B (human) ( 2 )
Treatments:
SB216763 ( 2 )

Downstream Regulation
Effects of modification on MAFA:
molecular association, regulation ( 2 , 3 ) , phosphorylation ( 4 ) , protein degradation ( 2 , 4 ) , ubiquitination ( 4 )
Effects of modification on biological processes:
transcription, induced ( 4 ) , transcription, inhibited ( 2 )
Induce interaction with:
MAFA (mouse) ( 3 ) , PSME3 (mouse) ( 2 )

References 

1

Iwaoka R, Kataoka K (2018) Glucose regulates MafA transcription factor abundance and insulin gene expression by inhibiting AMP-activated protein kinase in pancreatic β-cells. J Biol Chem
29348175   Curated Info

2

Kanai K, et al. (2011) Proteasome activator PA28{gamma} stimulates degradation of GSK3-phosphorylated insulin transcription activator MAFA. J Mol Endocrinol 47, 119-27
21646385   Curated Info

3

Guo S, Vanderford NL, Stein R (2010) Phosphorylation within the MafA N terminus regulates C-terminal dimerization and DNA binding. J Biol Chem 285, 12655-61
20208071   Curated Info

4

Guo S, et al. (2009) The stability and transactivation potential of the mammalian MafA transcription factor are regulated by serine 65 phosphorylation. J Biol Chem 284, 759-65
19004825   Curated Info