Ser1497
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Home > Phosphorylation Site Page: > Ser1497  -  BRCA1 (human)

Site Information
EPGVERssPsKCPsL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448730

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 , 7 ) , electrophoretic mobility shift ( 6 ) , immunoassay ( 3 ) , immunoprecipitation ( 1 , 4 , 7 ) , mass spectrometry ( 2 , 6 , 7 ) , mutation of modification site ( 3 , 4 , 6 , 7 ) , phospho-antibody ( 1 , 4 , 5 ) , phosphopeptide mapping ( 7 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
breast cancer ( 1 , 2 , 3 , 5 ) , breast adenocarcinoma ( 5 ) , breast ductal carcinoma ( 1 , 2 , 5 ) , breast cancer, triple negative ( 1 , 3 ) , lung cancer ( 3 , 4 ) , non-small cell lung cancer ( 3 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 3 ) , CDK2 (human) ( 7 )
Kinases, in vitro:
ATM (human) ( 6 ) , CDK1 (human) ( 4 ) , CDK2 (human) ( 7 )
Treatments:
cisplatin ( 4 ) , doxycycline ( 4 ) , ionizing_radiation ( 6 ) , RO-3306 ( 1 )

Downstream Regulation
Effects of modification on BRCA1:
intracellular localization ( 3 , 4 ) , molecular association, regulation ( 6 )
Effects of modification on biological processes:
cell growth, induced ( 3 )
Induce interaction with:
ATM (human) ( 6 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 3 )

References 

1

Chabalier-Taste C, et al. (2016) Polo-like kinase 1 mediates BRCA1 phosphorylation and recruitment at DNA double-strand breaks. Oncotarget 7, 2269-83
26745677   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Johnson N, et al. (2011) Compromised CDK1 activity sensitizes BRCA-proficient cancers to PARP inhibition. Nat Med 17, 875-82
21706030   Curated Info

4

Johnson N, et al. (2009) Cdk1 participates in BRCA1-dependent S phase checkpoint control in response to DNA damage. Mol Cell 35, 327-39
19683496   Curated Info

5

Okada S, Ouchi T (2003) Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization. J Biol Chem 278, 2015-20
12427729   Curated Info

6

Cortez D, Wang Y, Qin J, Elledge SJ (1999) Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science 286, 1162-6
10550055   Curated Info

7

Ruffner H, et al. (1999) BRCA1 is phosphorylated at serine 1497 in vivo at a cyclin-dependent kinase 2 phosphorylation site. Mol Cell Biol 19, 4843-54
10373534   Curated Info