Ser177

Site Information
EVGAGyNsEDEYEAA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 449291

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 4 , 5 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 3 ) , western blotting ( 3 )
Disease tissue studied:	anthrax infection ( 8 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 6 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 1 ) , brain ( 9 ) , CD4+ (T lymphocyte) ( 3 ) , CD8+ (T lymphocyte) ( 3 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , kidney ( 9 ) , liver ( 5 , 9 ) , lung ( 9 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 7 ) , MEF (fibroblast) ( 7 ) , pancreas ( 9 ) , spleen ( 8 , 9 ) , testis ( 9 )

Upstream Regulation
Treatments:	antibody ( 3 ) , IL-2 ( 3 ) , ionomycin ( 3 ) , phorbol_ester ( 3 ) , siRNA ( 3 ) , TGF-beta ( 3 )

References
1	Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 32 28852199 Curated Info
2	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
3	Rutz S, et al. (2015) Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature 518, 417-21 25470037 Curated Info
4	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
5	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
6	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
7	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
8	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
9	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info