Tyr53
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Home > Phosphorylation Site Page: > Tyr53  -  SPRY1 (mouse)

Site Information
AIRGSNEyTEGPSVA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454589

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 1 , 5 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
leukemia ( 1 ) , acute myelogenous leukemia ( 1 )
Relevant cell line - cell type - tissue:
C2C12-D (muscle cell) ( 4 ) , CD4+ (T lymphocyte) ( 5 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) ( 3 ) , UT-7 (myeloid) ( 1 )

Upstream Regulation
Regulatory protein:
EPOR (human) ( 1 )
Treatments:
EGF ( 3 ) , Epo ( 1 ) , PDGF ( 3 )

Downstream Regulation
Effects of modification on SPRY1:
activity, induced ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 1 )

References 

1

Sathyanarayana P, et al. (2012) Spry1 as a novel regulator of erythropoiesis, EPO/EPOR target, and suppressor of JAK2. Blood 119, 5522-31
22508938   Curated Info

2

Zhou J (2010) CST Curation Set: 9104; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

3

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

4

Guo A (2007) CST Curation Set: 2565; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Choi H, Cho SY, Schwartz RH, Choi K (2006) Dual effects of Sprouty1 on TCR signaling depending on the differentiation state of the T cell. J Immunol 176, 6034-45
16670312   Curated Info