Thr80
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Home > Phosphorylation Site Page: > Thr80  -  Calmodulin (rat)

Site Information
MARKMkDtDsEEEIR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448704

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 5 ) , mutation of modification site ( 1 , 2 )
Relevant cell line - cell type - tissue:
BAEC (endothelial) ( 3 ) , CHO (fibroblast) ( 1 ) , cochlea ( 2 ) , HEK293-A (epithelial) ( 1 ) , liver ( 5 ) , neuron ( 1 ) , oocyte ( 2 )

Upstream Regulation
Putative in vivo kinases:
CK2A1 (human) ( 1 , 2 )
Kinases, in vitro:
CK2A1 (cow) ( 4 ) , CK2A1 (human) ( 1 ) , CK2A1 (rabbit) ( 6 ) , CK2A1 (rat) ( 5 )
Putative upstream phosphatases:
PPP1CA (human) ( 1 )
Treatments:
calyculin_A ( 1 ) , TBB ( 1 ) , TBCA ( 1 )

Downstream Regulation
Effects of modification on Calmodulin:
activity, inhibited ( 2 , 5 ) , molecular association, regulation ( 1 )
Induce interaction with:
KCNQ4 (human) ( 1 )

References 

1

Kang S, Xu M, Cooper EC, Hoshi N (2014) Channel-anchored protein kinase CK2 and protein phosphatase 1 reciprocally regulate KCNQ2-containing M-channels via phosphorylation of calmodulin. J Biol Chem 289, 11536-44
24627475   Curated Info

2

Bildl W, et al. (2004) Protein kinase CK2 is coassembled with small conductance Ca(2+)-activated K+ channels and regulates channel gating. Neuron 43, 847-58
15363395   Curated Info

3

Greif DM, Sacks DB, Michel T (2004) Calmodulin phosphorylation and modulation of endothelial nitric oxide synthase catalysis. Proc Natl Acad Sci U S A 101, 1165-70
14736917   Curated Info

4

Joyal JL, Crimmins DL, Thoma RS, Sacks DB (1996) Identification of insulin-stimulated phosphorylation sites on calmodulin. Biochemistry 35, 6267-75
8639568   Curated Info

5

Quadroni M, James P, Carafoli E (1994) Isolation of phosphorylated calmodulin from rat liver and identification of the in vivo phosphorylation sites. J Biol Chem 269, 16116-22
8206911   Curated Info

6

Sacks DB, Davis HW, Crimmins DL, McDonald JM (1992) Insulin-stimulated phosphorylation of calmodulin. Biochem J 286 ( Pt 1), 211-6
1520270   Curated Info