Thr170
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Home > O-GlcNAc Site Page: > Thr170  -  CRYAB (rat)

Site Information
REEkPAVtAAPKK__   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 5281706

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 3 ) , 3H acetate labeling ( 3 ) , immunoprecipitation ( 1 , 3 ) , mass spectrometry ( 2 , 3 ) , modification-specific antibody ( 1 ) , mutation of modification site ( 1 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:
glioblastoma multiforme ( 3 ) , glioma ( 3 )
Relevant cell line - cell type - tissue:
brain ( 2 ) , COS (fibroblast) ( 1 ) , lens ( 3 ) , myocyte-heart ( 1 ) , U373 MG (glial) ( 3 )

Upstream Regulation
Treatments:
heat_shock ( 1 ) , PNGase F ( 3 )

Downstream Regulation
Effects of modification on CRYAB:
intracellular localization ( 1 ) , molecular association, regulation ( 1 ) , phosphorylation ( 1 ) , protein degradation ( 3 )
Induce interaction with:
HSP27 (human) ( 1 )

References 

1

Krishnamoorthy V, Donofrio AJ, Martin JL (2013) O-GlcNAcylation of αB-crystallin regulates its stress-induced translocation and cytoprotection. Mol Cell Biochem 379, 59-68
23543138   Curated Info

2

Wang Z, et al. (2010) Enrichment and site mapping of O-linked N-acetylglucosamine by a combination of chemical/enzymatic tagging, photochemical cleavage, and electron transfer dissociation mass spectrometry. Mol Cell Proteomics 9, 153-60
19692427   Curated Info

3

Roquemore EP, Chevrier MR, Cotter RJ, Hart GW (1996) Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin. Biochemistry 35, 3578-86
8639509   Curated Info