Ser643
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Home > Phosphorylation Site Page: > Ser643  -  HIF1A (human)

Site Information
kILIAsPsPTHIHkE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470106

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 3 , 4 , 6 ) , mutation of modification site ( 2 , 7 , 8 ) , phospho-antibody ( 7 , 8 ) , western blotting ( 2 , 7 , 8 )
Disease tissue studied:
breast cancer, surrounding tissue ( 1 ) , colorectal cancer ( 2 ) , colorectal carcinoma ( 2 ) , liver cancer ( 7 ) , lung cancer ( 6 ) , non-small cell lung cancer ( 6 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
ERK2 (human) ( 7 ) , ERK2 (mouse) ( 8 )
Treatments:
PD98059 ( 8 )

Downstream Regulation
Effects of modification on HIF1A:
activity, induced ( 7 ) , intracellular localization ( 7 , 8 ) , molecular association, regulation ( 2 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
transcription, altered ( 7 ) , transcription, induced ( 2 , 8 )
Induce interaction with:
PIN1 (human) ( 2 )
Inhibit interaction with:
Exportin-1 (human) ( 7 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Han HJ, et al. (2016) Peptidyl Prolyl Isomerase PIN1 Directly Binds to and Stabilizes Hypoxia-Inducible Factor-1α. PLoS One 11, e0147038
26784107   Curated Info

3

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

7

Mylonis I, Chachami G, Paraskeva E, Simos G (2008) Atypical CRM1-dependent nuclear export signal mediates regulation of hypoxia-inducible factor-1alpha by MAPK. J Biol Chem 283, 27620-7
18687685   Curated Info

8

Mylonis I, et al. (2006) Identification of MAPK phosphorylation sites and their role in the localization and activity of hypoxia-inducible factor-1alpha. J Biol Chem 281, 33095-106
16954218   Curated Info