Ser641
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Home > Phosphorylation Site Page: > Ser641  -  HIF1A (human)

Site Information
DIkILIAsPsPTHIH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470105

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 , 4 , 5 ) , phospho-antibody ( 4 , 5 ) , western blotting ( 1 , 4 , 5 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , liver cancer ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 ) , 293T (epithelial) ( 4 ) , HCT116 (intestinal) ( 1 ) , HeLa (cervical) ( 2 , 3 , 4 , 5 ) , Huh7 (hepatic) ( 4 )

Upstream Regulation
Kinases, in vitro:
ERK2 (human) ( 4 ) , ERK2 (mouse) ( 5 )
Treatments:
PD98059 ( 5 )

Downstream Regulation
Effects of modification on HIF1A:
activity, induced ( 4 ) , intracellular localization ( 4 , 5 ) , molecular association, regulation ( 1 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
transcription, altered ( 4 ) , transcription, induced ( 1 , 5 )
Induce interaction with:
PIN1 (human) ( 1 )
Inhibit interaction with:
Exportin-1 (human) ( 4 )

References 

1

Han HJ, et al. (2016) Peptidyl Prolyl Isomerase PIN1 Directly Binds to and Stabilizes Hypoxia-Inducible Factor-1α. PLoS One 11, e0147038
26784107   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

4

Mylonis I, Chachami G, Paraskeva E, Simos G (2008) Atypical CRM1-dependent nuclear export signal mediates regulation of hypoxia-inducible factor-1alpha by MAPK. J Biol Chem 283, 27620-7
18687685   Curated Info

5

Mylonis I, et al. (2006) Identification of MAPK phosphorylation sites and their role in the localization and activity of hypoxia-inducible factor-1alpha. J Biol Chem 281, 33095-106
16954218   Curated Info