Ser342
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Home > Phosphorylation Site Page: > Ser342  -  MDM4 (human)

Site Information
SkLTHSLsTSDITAI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470031

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 9 ) , mass spectrometry ( 1 , 3 , 6 , 12 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 2 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , peptide sequencing ( 12 ) , phospho-antibody ( 12 ) , western blotting ( 2 , 7 , 9 , 10 , 12 )
Disease tissue studied:
bone cancer ( 7 , 8 , 9 , 12 ) , breast cancer ( 12 ) , colorectal cancer ( 12 ) , colorectal carcinoma ( 12 ) , lung cancer ( 2 , 7 , 9 ) , non-small cell lung cancer ( 2 , 7 , 9 ) , neuroblastoma ( 9 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
PPM1D (human) ( 8 )
Putative in vivo kinases:
Chk2 (human) ( 12 )
Kinases, in vitro:
AMPKA1 (rat) ( 2 ) , Chk1 (human) ( 12 ) , Chk2 (human) ( 11 , 12 )

Downstream Regulation
Effects of modification on MDM4:
intracellular localization ( 9 , 11 ) , molecular association, regulation ( 2 , 8 , 9 , 11 ) , protein degradation ( 7 , 8 , 12 , 13 ) , ubiquitination ( 7 , 8 , 9 )
Induce interaction with:
14-3-3 gamma (human) ( 2 ) , 14-3-3 theta (human) ( 11 ) , MDM2 (human) ( 8 )
Inhibit interaction with:
MDM2 (human) ( 9 ) , USP7 (human) ( 8 ) , p53 (human) ( 9 )

References 

1

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

2

He G, et al. (2014) AMP-Activated Protein Kinase Induces p53 by Phosphorylating MDMX and Inhibiting Its Activity. Mol Cell Biol 34, 148-57
24190973   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Lu Z, et al. (2013) Protein phosphatase 1 inhibits p53 signaling by dephosphorylating and stabilizing Mdmx. Cell Signal 25, 796-804
23277204   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Li X, et al. (2012) Abnormal MDMX degradation in tumor cells due to ARF deficiency. Oncogene 31, 3721-32
22120712   Curated Info

8

Zhang X, et al. (2009) Phosphorylation and degradation of MdmX is inhibited by Wip1 phosphatase in the DNA damage response. Cancer Res 69, 7960-8
19808970   Curated Info

9

Ohtsubo C, et al. (2009) Cytoplasmic tethering is involved in synergistic inhibition of p53 by Mdmx and Mdm2. Cancer Sci 100, 1291-9
19432880   Curated Info

10

Zuckerman V, et al. (2009) c-Abl phosphorylates Hdmx and regulates its interaction with p53. J Biol Chem 284, 4031-9
19075013   Curated Info

11

Pereg Y, et al. (2006) Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage. Mol Cell Biol 26, 6819-31
16943424   Curated Info

12

Chen L, et al. (2005) ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage. EMBO J 24, 3411-22
16163388   Curated Info

13

Pereg Y, et al. (2005) Phosphorylation of Hdmx mediates its Hdm2- and ATM-dependent degradation in response to DNA damage. Proc Natl Acad Sci U S A 102, 5056-61
15788536   Curated Info