Ser331
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser331  -  TXA2-R (human)

Site Information
sTRPRsLsLQPQLtQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470029

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 1 , 2 , 4 , 6 , 7 , 8 , 9 , 10 , 11 ) , mutation of modification site ( 3 , 12 , 13 ) , phospho-antibody ( 3 , 13 ) , western blotting ( 3 , 12 , 13 )
Disease tissue studied:
breast cancer ( 2 ) , breast ductal carcinoma ( 2 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 , 2 ) , leukemia ( 8 ) , acute myelogenous leukemia ( 8 ) , lung cancer ( 4 , 6 ) , non-small cell lung cancer ( 6 ) , non-small cell lung adenocarcinoma ( 4 ) , ovarian cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 3 ) , PKG1 (human) ( 12 )
Kinases, in vitro:
PKCA (human) ( 13 )
Treatments:
anti-CD3 ( 7 ) , ischemia ( 2 ) , KT5823 ( 12 ) , L-NAME ( 12 ) , LY 83583 ( 12 ) , phorbol_ester ( 13 ) , U46619 ( 12 )

Downstream Regulation
Effects of modification on TXA2-R:
activity, induced ( 3 ) , molecular association, regulation ( 3 ) , receptor desensitization, altered ( 13 ) , receptor desensitization, induced ( 12 )
Effects of modification on biological processes:
signaling pathway regulation ( 12 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Santhosh KT, et al. (2014) Thromboxane receptor hyper-responsiveness in hypoxic pulmonary hypertension requires serine 324. Br J Pharmacol 171, 676-87
24490858   Curated Info

4

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

7

Ruperez P, Gago-Martinez A, Burlingame AL, Oses-Prieto JA (2012) Quantitative phosphoproteomic analysis reveals a role for serine and threonine kinases in the cytoskeletal reorganization in early T cell receptor activation in human primary T cells. Mol Cell Proteomics 11, 171-86
22499768   Curated Info

8

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

9

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

10

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

11

Thingholm TE, et al. (2008) TiO2-Based Phosphoproteomic Analysis of the Plasma Membrane and the Effects of Phosphatase Inhibitor Treatment. J Proteome Res 7, 3304-3313
18578522   Curated Info

12

Kelley-Hickie LP, O'Keeffe MB, Reid HM, Kinsella BT (2007) Homologous desensitization of signalling by the alpha (alpha) isoform of the human thromboxane A2 receptor: a specific role for nitric oxide signalling. Biochim Biophys Acta 1773, 970-89
17466390   Curated Info

13

Yan FX, et al. (2002) Serine 331 is major site of phosphorylation and desensitization induced by protein kinase C in thromboxane receptor alpha. Acta Pharmacol Sin 23, 952-60
12370102   Curated Info