Ser635
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Home > Phosphorylation Site Page: > Ser635  -  IRS1 (rat)

Site Information
yMPMsPKsVsAPQQI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447507

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 5 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 ) , western blotting ( 1 , 3 , 4 , 5 )
Disease tissue studied:
liver cancer ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 ) , 3T3-L1 (fibroblast) ( 5 ) , C2C12 (myoblast) ( 4 ) , granulosa ( 1 ) , H9c2 (myoblast) ( 4 ) , HepG2 (hepatic) ( 5 ) , L6 (myoblast) ( 5 ) , myoblast ( 3 ) , RALA255-10G (hepatocyte) ( 6 )

Upstream Regulation
Regulatory protein:
LKB1 (human) ( 5 ) , RHEB (human) ( 5 )
Putative in vivo kinases:
ROCK1 (rat) ( 4 )
Kinases, in vitro:
mTOR (human) ( 5 )
Treatments:
2-deoxyglucose ( 5 ) , glucose ( 5 ) , insulin ( 3 , 5 , 6 ) , leucine ( 5 ) , metformin ( 5 ) , rapamycin ( 5 ) , serum_withdrawal ( 4 ) , siRNA ( 5 ) , testosterone ( 3 ) , TNF ( 5 ) , Y27632 ( 4 )

Downstream Regulation
Effects of modification on IRS1:
molecular association, regulation ( 5 )
Inhibit interaction with:
PIK3R1 (rat) ( 5 )

References 

1

Law NC, White MF, Hunzicker-Dunn ME (2016) G protein-coupled receptors (GPCRs) That Signal via Protein Kinase A (PKA) Cross-talk at Insulin Receptor Substrate 1 (IRS1) to Activate the phosphatidylinositol 3-kinase (PI3K)/AKT Pathway. J Biol Chem 291, 27160-27169
27856640   Curated Info

2

Rajesh P, Balasubramanian K (2014) Phthalate exposure in utero causes epigenetic changes and impairs insulin signalling. J Endocrinol 223, 47-66
25232145   Curated Info

3

Allemand MC, et al. (2009) Effect of testosterone on insulin stimulated IRS1 Ser phosphorylation in primary rat myotubes--a potential model for PCOS-related insulin resistance. PLoS One 4, e4274
19169352   Curated Info

4

Lim MJ, et al. (2007) RhoA/Rho kinase blocks muscle differentiation via serine phosphorylation of insulin receptor substrate-1 and -2. Mol Endocrinol 21, 2282-93
17579208   Curated Info

5

Tzatsos A, Kandror KV (2006) Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation. Mol Cell Biol 26, 63-76
16354680   Curated Info

6

Schattenberg JM, et al. (2005) Hepatocyte CYP2E1 overexpression and steatohepatitis lead to impaired hepatic insulin signaling. J Biol Chem 280, 9887-94
15632182   Curated Info