Ser71
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Home > Phosphorylation Site Page: > Ser71  -  NCCT (mouse)

Site Information
AYEHYANsALPGEPR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469631

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 8 ) , mutation of modification site ( 4 , 7 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 9 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 9 )
Relevant cell line - cell type - tissue:
'kidney, cortex' ( 5 ) , COS (fibroblast) ( 4 ) , COS7 (fibroblast) ( 7 ) , kidney ( 1 , 2 , 3 , 6 , 8 , 9 )

Upstream Regulation
Regulatory protein:
KLHL3 (mouse) ( 1 ) , STLK3 (mouse) ( 6 ) , WNK4 (mouse) ( 6 )
Treatments:
HET0016 ( 2 ) , high-potassium diet ( 9 ) , high-salt diet ( 9 ) , low-potassium diet ( 9 ) , low-salt diet ( 6 , 9 )

Downstream Regulation
Effects of modification on NCCT:
activity, induced ( 5 ) , ubiquitination ( 7 )

References 

1

Sasaki E, et al. (2017) KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3. Mol Cell Biol 37
28052936   Curated Info

2

Savas Ü, et al. (2016) 20-Hydroxyeicosatetraenoic Acid (HETE)-dependent Hypertension in Human Cytochrome P450 (CYP) 4A11 Transgenic Mice: NORMALIZATION OF BLOOD PRESSURE BY SODIUM RESTRICTION, HYDROCHLOROTHIAZIDE, OR BLOCKADE OF THE TYPE 1 ANGIOTENSIN II RECEPTOR. J Biol Chem 291, 16904-19
27298316   Curated Info

3

Ivy JR, et al. (2016) Glucocorticoids Induce Nondipping Blood Pressure by Activating the Thiazide-Sensitive Cotransporter. Hypertension 67, 1029-37
26953322   Curated Info

4

Wang J, et al. (2015) Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter. Nat Med 21, 820-6
26099046   Curated Info

5

Arystarkhova E, et al. (2014) Paradoxical activation of the sodium chloride cotransporter (NCC) without hypertension in kidney deficient in a regulatory subunit of Na,K-ATPase, FXYD2. Physiol Rep 2
25472608   Curated Info

6

Yang SS, et al. (2013) Phosphorylation regulates NCC stability and transporter activity in vivo. J Am Soc Nephrol 24, 1587-97
23833262   Curated Info

7

Hossain Khan MZ, et al. (2012) Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. Biochem Biophys Res Commun 425, 456-61
22846565   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Vallon V, et al. (2009) Expression and phosphorylation of the Na+-Cl- cotransporter NCC in vivo is regulated by dietary salt, potassium, and SGK1. Am J Physiol Renal Physiol 297, F704-12
19570885   Curated Info