Ser130
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Home > Phosphorylation Site Page: > Ser130  -  DARPP-32 (mouse)

Site Information
EEDEEEDsQAEVLKG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448046

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 12 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 8 ) , phospho-antibody ( 6 , 7 , 8 , 9 , 10 , 11 , 13 ) , western blotting ( 6 , 7 , 10 )
Disease tissue studied:
neuroblastoma ( 10 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 12 ) , 'brain, embryonic' ( 3 ) , 'brain, neostriatum' ( 9 , 10 , 13 ) , 'brain, striatum' ( 6 , 7 , 8 , 11 , 12 ) , brain ( 1 , 2 , 4 , 5 ) , Neuro-2a (neuron) ( 10 ) , neuron-'brain, hippocampus' ( 11 ) , neuron:synaptosome-'brain, cerebral cortex' ( 11 )

Upstream Regulation
Putative in vivo kinases:
CK1E (mouse) ( 10 , 13 )
Treatments:
5-HT ( 12 ) , 5-HT2_receptor_agonists ( 12 ) , 5-HT4_receptor_agonists ( 12 ) , 5-HT5_receptor_agonists ( 12 ) , alpha-BTX ( 9 ) , BAPTA-AM ( 10 ) , butyrolactone ( 13 ) , caffeine ( 7 ) , ciclosporin ( 10 ) , CK1-7 ( 13 ) , CNQX ( 9 ) , cocaine ( 8 ) , DHbetaE ( 9 ) , DHPG ( 10 , 13 ) , fluoxetine ( 11 ) , IC261 ( 13 ) , ionomycin ( 10 ) , isoflurane ( 6 ) , ketamine ( 6 ) , mecamylamine ( 9 ) , MK801 ( 9 ) , nicotine ( 9 ) , propofol ( 6 ) , raclopride ( 9 ) , SCH_23390 ( 9 ) , TTX ( 9 ) , U73122 ( 10 ) , ZM241385 ( 10 )

Downstream Regulation
Effects of modification on DARPP-32:
activity, induced ( 13 )

References 

1

Wiƛniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

2

Zhou J (2009) CST Curation Set: 7431; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSP
Curated Info

3

Zhou J (2009) CST Curation Set: 7381; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: QXp[ST]
Curated Info

4

Zhou J (2009) CST Curation Set: 7383; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: QXp[ST]
Curated Info

5

Zhou J (2009) CST Curation Set: 7384; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: QXp[ST]
Curated Info

6

Snyder GL, Galdi S, Hendrick JP, Hemmings HC (2007) General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53, 619-30
17826804   Curated Info

7

Sahin B, et al. (2007) Evaluation of neuronal phosphoproteins as effectors of caffeine and mediators of striatal adenosine A2A receptor signaling. Brain Res 1129, 1-14
17157277   Curated Info

8

Zhang Y, et al. (2006) Cocaine self-administration in mice is inversely related to phosphorylation at Thr34 (protein kinase A site) and Ser130 (kinase CK1 site) of DARPP-32. J Neurosci 26, 2645-51
16525043   Curated Info

9

Hamada M, et al. (2005) Nicotine regulates DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of 32 kDa) phosphorylation at multiple sites in neostriatal neurons. J Pharmacol Exp Ther 315, 872-8
16040813   Curated Info

10

Liu F, Virshup DM, Nairn AC, Greengard P (2002) Mechanism of regulation of casein kinase I activity by group I metabotropic glutamate receptors. J Biol Chem 277, 45393-9
12223474   Curated Info

11

Svenningsson P, et al. (2002) Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac). Proc Natl Acad Sci U S A 99, 3182-7
11880651   Curated Info

12

Svenningsson P, et al. (2002) DARPP-32 mediates serotonergic neurotransmission in the forebrain. Proc Natl Acad Sci U S A 99, 3188-93
11880652   Curated Info

13

Liu F, et al. (2001) Regulation of cyclin-dependent kinase 5 and casein kinase 1 by metabotropic glutamate receptors. Proc Natl Acad Sci U S A 98, 11062-8
11572969   Curated Info