Ser660

Site Information
PAHPtPLsRSAsLsY SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 5207727

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 5 , 6 , 7 , 9 , 10 )
Disease tissue studied:	breast cancer ( 6 ) , luminal B breast cancer ( 1 ) , lung cancer ( 6 , 9 ) , non-small cell lung cancer ( 6 )
Relevant cell line - cell type - tissue:	breast ( 1 ) , BT-20 (breast cell) ( 6 ) , CL1-0 (pulmonary) ( 9 ) , CL1-1 (pulmonary) ( 9 ) , CL1-2 (pulmonary) ( 9 ) , CL1-5 (pulmonary) ( 9 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 7 ) , Flp-In T-Rex-293 (epithelial) ( 7 ) , HCC1937 (breast cell) ( 6 ) , HeLa (cervical) ( 5 ) , Jurkat (T lymphocyte) ( 3 ) , K562 (erythroid) ( 5 ) , MCF-7 (breast cell) ( 6 ) , MDA-MB-468 (breast cell) ( 6 ) , NCI-H1568 (pulmonary) ( 6 ) , NCI-H3255 (pulmonary) ( 10 ) , NCI-H647 (pulmonary) ( 6 )

Upstream Regulation
Treatments:	metastatic potential ( 9 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
3	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
4	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
5	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
6	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
7	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
8	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
9	Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97 20815410 Curated Info
10	Moritz A (2008) CST Curation Set: 5650; Year: 2008; Biosample/Treatment: cell line, NCI-H3255/rapamycin; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS/T) Immunoaffinity Beads Cat#: 1979 Curated Info