Thr249

Site Information
DNGsPAMtHRNLtss SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 5196395

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 )
Disease tissue studied:	gastric cancer ( 3 , 4 ) , gastric carcinoma ( 3 , 4 )
Relevant cell line - cell type - tissue:	Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 1 ) , Flp-In T-Rex-293 (epithelial) ( 1 ) , Jurkat (T lymphocyte) ( 2 ) , MKN-45 (gastric) ( 3 , 4 )

References
1	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
2	Possemato A (2010) CST Curation Set: 9615; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Curated Info
3	Moritz A, et al. (2010) Akt-RSK-S6 Kinase Signaling Networks Activated by Oncogenic Receptor Tyrosine Kinases. Sci Signal 3, ra64 20736484 Curated Info
4	Rush J (2008) CST Curation Set: 4483; Year: 2008; Biosample/Treatment: cell line, MKN-45/untreated &'\|\|' normal; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info