Ser24
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Home > Phosphorylation Site Page: > Ser24  -  TNNI3 (human)

Site Information
APIRRRssNyRAyAt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448424

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 16 ) , immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 14 , 17 , 18 , 19 , 20 , 21 , 24 , 25 , 26 , 27 , 28 ) , mass spectrometry (in vitro) ( 13 , 16 ) , mutation of modification site ( 1 , 4 , 7 , 9 , 10 , 13 , 15 , 16 ) , phospho-antibody ( 1 , 2 , 5 , 6 , 8 , 9 , 11 , 12 , 15 , 22 ) , western blotting ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 15 , 22 )
Disease tissue studied:
atrial fibrillation ( 8 ) , ventricular tachycardia ( 8 , 17 , 18 , 19 , 20 , 21 ) , DCM ( 10 )
Relevant cell line - cell type - tissue:
'heart, ventricle' ( 9 ) , 293 (epithelial) ( 1 , 5 ) , ARVM (myocyte) ( 2 ) , cardiac-heart ( 3 ) , E.coli (bacterial) ( 4 , 10 ) , heart ( 8 , 11 , 14 , 15 , 17 , 18 , 19 , 20 , 21 , 24 , 27 , 28 ) , myocyte-heart ( 1 , 6 , 7 , 10 , 12 , 16 , 22 )

Upstream Regulation
Regulatory protein:
Src (human) ( 1 )
Kinases, in vitro:
AMPKA1 (human) ( 16 ) , PKACA (human) ( 3 , 22 , 30 , 31 ) , PKCA (human) ( 29 ) , PKCD (human) ( 23 )
Putative upstream phosphatases:
PPP2CA (human) ( 6 )
Treatments:
5-HT ( 8 ) , acadesine ( 16 ) , colforsin ( 8 ) , compound_C ( 16 ) , heart failure ( 11 ) , ischemia ( 9 ) , isoproterenol ( 6 ) , norepinephrine ( 8 ) , racepinefrine ( 8 )

Downstream Regulation
Effects of modification on TNNI3:
activity, induced ( 10 ) , activity, inhibited ( 13 ) , molecular association, regulation ( 4 , 29 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 3 , 31 )
Inhibit interaction with:
TNNC1 (human) ( 4 , 29 )

Disease / Diagnostics Relevance
Relevant diseases:
cardiomyopathy ( 12 ) , DCM ( 14 )

References 

1

Gong J, Park M, Steinberg SF (2017) CLEAVAGE ALTERS THE MOLECULAR DETERMINANTS OF PROTEIN KINASE C-δ CATALYTIC ACTIVITY. Mol Cell Biol
28784722   Curated Info

2

Weeks KL, et al. (2017) β-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55α-PP2A-Mediated Dephosphorylation. J Am Heart Assoc 6
28343149   Curated Info

3

Dvornikov AV, et al. (2016) Restrictive Cardiomyopathy Troponin I R145W Mutation Does Not Perturb Myofilament Length-dependent Activation in Human Cardiac Sarcomeres. J Biol Chem 291, 21817-21828
27557662   Curated Info

4

Salhi HE, et al. (2016) Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration. Front Physiol 7, 567
28018230   Curated Info

5

Gong J, et al. (2015) The C2 Domain and Altered ATP-Binding Loop Phosphorylation at Ser³⁵⁹ Mediate the Redox-Dependent Increase in Protein Kinase C-δ Activity. Mol Cell Biol 35, 1727-40
25755284   Curated Info

6

Kirchhefer U, et al. (2014) Cardiac Function Is Regulated by B56α-mediated Targeting of Protein Phosphatase 2A (PP2A) to Contractile Relevant Substrates. J Biol Chem 289, 33862-73
25320082   Curated Info

7

Salhi HE, et al. (2014) Cardiac troponin I tyrosine 26 phosphorylation decreases myofilament Ca2+ sensitivity and accelerates deactivation. J Mol Cell Cardiol 76, 257-64
25252176   Curated Info

8

Christ T, et al. (2014) Arrhythmias, elicited by catecholamines and serotonin, vanish in human chronic atrial fibrillation. Proc Natl Acad Sci U S A 111, 11193-8
25024212   Curated Info

9

Nixon BR, et al. (2014) Combined troponin I Ser-150 and Ser-23/24 phosphorylation sustains thin filament Ca(2+) sensitivity and accelerates deactivation in an acidic environment. J Mol Cell Cardiol 72, 177-85
24657721   Curated Info

10

Wijnker PJ, et al. (2014) Length-dependent activation is modulated by cardiac troponin I bisphosphorylation at Ser23 and Ser24 but not by Thr143 phosphorylation. Am J Physiol Heart Circ Physiol 306, H1171-81
24585778   Curated Info

11

Han YS, Arroyo J, Ogut O (2013) Human heart failure is accompanied by altered protein kinase A subunit expression and post-translational state. Arch Biochem Biophys 538, 25-33
23942052   Curated Info

12

Hamdani N, et al. (2013) Deranged myofilament phosphorylation and function in experimental heart failure with preserved ejection fraction. Cardiovasc Res 97, 464-71
23213108   Curated Info

13

Kooij V, et al. (2013) PKCα-Specific Phosphorylation of the Troponin Complex in Human Myocardium: A Functional and Proteomics Analysis. PLoS One 8, e74847
24116014   Curated Info

14

Zhang P, et al. (2012) Multiple reaction monitoring to identify site-specific troponin I phosphorylated residues in the failing human heart. Circulation 126, 1828-37
22972900   Curated Info

15

Nixon BR, et al. (2012) AMP-activated Protein Kinase Phosphorylates Cardiac Troponin I at Ser-150 to Increase Myofilament Calcium Sensitivity and Blunt PKA-dependent Function. J Biol Chem 287, 19136-47
22493448   Curated Info

16

Oliveira SM, et al. (2012) AMP-activated protein kinase phosphorylates cardiac troponin I and alters contractility of murine ventricular myocytes. Circ Res 110, 1192-201
22456184   Curated Info

17

Li Y (2011) CST Curation Set: 10514; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

18

Li Y (2011) CST Curation Set: 10516; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

19

Li Y (2011) CST Curation Set: 10517; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

20

Li Y (2011) CST Curation Set: 10527; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

21

Li Y (2011) CST Curation Set: 10446; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

22

Kooij V, et al. (2010) Effect of troponin I Ser23/24 phosphorylation on Ca2+-sensitivity in human myocardium depends on the phosphorylation background. J Mol Cell Cardiol 48, 954-63
20079747   Curated Info

23

Rybin VO, et al. (2009) Regulatory autophosphorylation sites on protein kinase C-delta at threonine-141 and threonine-295. Biochemistry 48, 4642-51
19366211   Curated Info

24

Zabrouskov V, Ge Y, Schwartz J, Walker JW (2008) Unraveling molecular complexity of phosphorylated human cardiac troponin I by top down electron capture dissociation/electron transfer dissociation mass spectrometry. Mol Cell Proteomics 7, 1838-49
18445579   Curated Info

25

Stokes M (2008) CST Curation Set: 5164; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

26

Tucker M (2008) CST Curation Set: 4516; Year: 2008; Biosample/Treatment: cell line, EU-11/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

27

Stokes M (2008) CST Curation Set: 4142; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

28

Stokes M (2008) CST Curation Set: 4143; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

29

Ward DG, et al. (2004) NMR and mutagenesis studies on the phosphorylation region of human cardiac troponin I. Biochemistry 43, 5772-81
15134451   Curated Info

30

Ward DG, Ashton PR, Trayer HR, Trayer IP (2001) Additional PKA phosphorylation sites in human cardiac troponin I. Eur J Biochem 268, 179-85
11121119   Curated Info

31

Dohet C, al-Hillawi E, Trayer IP, Rüegg JC (1995) Reconstitution of skinned cardiac fibres with human recombinant cardiac troponin-I mutants and troponin-C. FEBS Lett 377, 131-4
8543035   Curated Info