Ser71
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Home > Phosphorylation Site Page: > Ser71  -  NCCT (rat)

Site Information
AYEHYANsALPGEPR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469631

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 ) , mutation of modification site ( 1 , 5 ) , phospho-antibody ( 2 , 4 ) , western blotting ( 2 , 4 )
Disease tissue studied:
kidney cancer ( 4 )
Relevant cell line - cell type - tissue:
'kidney, cortex' ( 3 ) , kidney ( 4 ) , MDCK (epithelial) ( 1 ) , oocyte [CPEB (mouse)] ( 5 ) , renal tubule cell ( 2 )

Upstream Regulation
Treatments:
ciclosporin ( 2 ) , desmopressin ( 4 ) , low_Ca(2+) ( 5 )

Downstream Regulation
Effects of modification on NCCT:
receptor internalization, inhibited ( 1 ) , ubiquitination ( 1 )

References 

1

Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis. J Biol Chem 289, 13347-61
24668812   Curated Info

2

Melnikov S, et al. (2011) Cyclosporine metabolic side effects: association with the WNK4 system. Eur J Clin Invest 41, 1113-20
21434893   Curated Info

3

Feric M, et al. (2011) Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule. Am J Physiol Cell Physiol 300, C755-70
21209370   Curated Info

4

Mutig K, et al. (2010) Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation. Am J Physiol Renal Physiol 298, F502-9
20007345   Curated Info

5

Pacheco-Alvarez D, et al. (2006) The Na+:Cl- cotransporter is activated and phosphorylated at the amino-terminal domain upon intracellular chloride depletion. J Biol Chem 281, 28755-63
16887815   Curated Info