Thr60
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Home > Phosphorylation Site Page: > Thr60  -  NCCT (human)

Site Information
MRtFGYNtIDVVPtY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469617

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 6 ) , mass spectrometry ( 6 ) , mutation of modification site ( 5 ) , phospho-antibody ( 1 , 2 , 3 , 6 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 6 ) , kidney ( 3 ) , oocyte ( 5 ) , urine ( 1 )

Upstream Regulation
Kinases, in vitro:
OSR1 (human) ( 4 , 6 ) , STLK3 (human) ( 6 )
Treatments:
ciclosporin ( 3 ) , hydrochlorothiazide ( 2 ) , hypotonic_buffer ( 6 ) , valsartan ( 2 )

Downstream Regulation
Effects of modification on NCCT:
activity, induced ( 6 ) , molecular association, regulation ( 5 )
Inhibit interaction with:
ADD3 (human) ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
pre-ecalampsia ( 1 )

References 

1

Hu CC, et al. (2018) Pre-eclampsia is associated with altered expression of the renal sodium transporters NKCC2, NCC and ENaC in urinary extracellular vesicles. PLoS One 13, e0204514
30248150   Curated Info

2

Pathare G, et al. (2017) Hydrochlorothiazide treatment increases the abundance of the NaCl cotransporter in urinary extracellular vesicles of essential hypertensive patients. Am J Physiol Renal Physiol 312, F1063-F1072
28274929   Curated Info

3

Tutakhel OAZ, et al. (2017) NaCl cotransporter abundance in urinary vesicles is increased by calcineurin inhibitors and predicts thiazide sensitivity. PLoS One 12, e0176220
28430812   Curated Info

4

Filippi BM, et al. (2011) MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases. EMBO J 30, 1730-41
21423148   Curated Info

5

Dimke H, et al. (2011) γ-Adducin stimulates the thiazide-sensitive NaCl cotransporter. J Am Soc Nephrol 22, 508-17
21164023   Curated Info

6

Richardson C, et al. (2008) Activation of the thiazide-sensitive Na+-Cl- cotransporter by the WNK-regulated kinases SPAK and OSR1. J Cell Sci 121, 675-84
18270262   Curated Info