Thr55
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr55  -  NCCT (human)

Site Information
SStFCMRtFGYNtID   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469616

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 5 ) , mass spectrometry ( 5 ) , mutation of modification site ( 4 ) , phospho-antibody ( 1 , 2 , 5 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 ) , oocyte ( 2 , 4 )

Upstream Regulation
Putative in vivo kinases:
WNK4 (human) ( 2 )
Kinases, in vitro:
OSR1 (human) ( 3 , 5 ) , STLK3 (human) ( 5 )
Treatments:
hydrochlorothiazide ( 1 ) , hypotonic_buffer ( 5 ) , valsartan ( 1 )

Downstream Regulation
Effects of modification on NCCT:
molecular association, regulation ( 4 )
Inhibit interaction with:
ADD3 (human) ( 4 )

References 

1

Pathare G, et al. (2017) Hydrochlorothiazide treatment increases the abundance of the NaCl cotransporter in urinary extracellular vesicles of essential hypertensive patients. Am J Physiol Renal Physiol 312, F1063-F1072
28274929   Curated Info

2

Bazúa-Valenti S, et al. (2015) The Effect of WNK4 on the Na+-Cl- Cotransporter Is Modulated by Intracellular Chloride. J Am Soc Nephrol 26, 1781-6
25542968   Curated Info

3

Filippi BM, et al. (2011) MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases. EMBO J 30, 1730-41
21423148   Curated Info

4

Dimke H, et al. (2011) γ-Adducin stimulates the thiazide-sensitive NaCl cotransporter. J Am Soc Nephrol 22, 508-17
21164023   Curated Info

5

Richardson C, et al. (2008) Activation of the thiazide-sensitive Na+-Cl- cotransporter by the WNK-regulated kinases SPAK and OSR1. J Cell Sci 121, 675-84
18270262   Curated Info