Thr53
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr53  -  NCCT (rat)

Site Information
GStLYLRtFGYNtID   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469616

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 5 ) , mutation of modification site ( 2 , 4 , 9 ) , phospho-antibody ( 1 , 3 , 4 , 6 , 7 , 8 ) , western blotting ( 1 , 3 , 4 , 6 , 7 , 8 )
Disease tissue studied:
kidney cancer ( 7 , 8 ) , obesity, hyperphagic ( 3 )
Relevant cell line - cell type - tissue:
'kidney, cortex' ( 3 ) , 'kidney, tubule' ( 6 ) , Flp-In T-Rex-293 (epithelial) ( 3 ) , kidney ( 4 , 5 , 7 , 8 ) , MDCK (epithelial) ( 2 ) , oocyte [CPEB (mouse)] ( 9 ) , peritoneum ( 1 )

Upstream Regulation
Treatments:
aldosterone ( 6 ) , amiloride ( 1 ) , angiotensin_2 ( 6 ) , desmopressin ( 7 , 8 ) , furosemide ( 1 ) , insulin ( 3 ) , low_Ca(2+) ( 9 ) , LY294002 ( 3 )

Downstream Regulation
Effects of modification on NCCT:
activity, induced ( 3 , 4 ) , intracellular localization ( 7 ) , receptor internalization, inhibited ( 2 ) , ubiquitination ( 2 )

References 

1

Frindt G, et al. (2017) Responses of distal nephron Na(+) transporters to acute volume depletion and hyperkalemia. Am J Physiol Renal Physiol 313, F62-F73
28356292   Curated Info

2

Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis. J Biol Chem 289, 13347-61
24668812   Curated Info

3

Komers R, et al. (2012) Enhanced phosphorylation of Na+-Cl- co-transporter in experimental metabolic syndrome: role of insulin. Clin Sci (Lond) 123, 635-47
22651238   Curated Info

4

Rosenbaek LL, et al. (2012) Characterization of a novel phosphorylation site in the sodium-chloride cotransporter, NCC. J Physiol 590, 6121-39
22966159   Curated Info

5

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

6

van der Lubbe N, et al. (2011) Angiotensin II induces phosphorylation of the thiazide-sensitive sodium chloride cotransporter independent of aldosterone. Kidney Int 79, 66-76
20720527   Curated Info

7

Pedersen NB, et al. (2010) Vasopressin induces phosphorylation of the thiazide-sensitive sodium chloride cotransporter in the distal convoluted tubule. Kidney Int 78, 160-9
20445498   Curated Info

8

Mutig K, et al. (2010) Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation. Am J Physiol Renal Physiol 298, F502-9
20007345   Curated Info

9

Pacheco-Alvarez D, et al. (2006) The Na+:Cl- cotransporter is activated and phosphorylated at the amino-terminal domain upon intracellular chloride depletion. J Biol Chem 281, 28755-63
16887815   Curated Info