Ser295
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Home > Phosphorylation Site Page: > Ser295  -  PSD-95 (rat)

Site Information
PTsPRRYsPVAKDLL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456936

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 5 ) , mutation of modification site ( 1 , 4 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 , 4 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 2 ) , 'brain, cerebral cortex' ( 2 ) , 'brain, forebrain' ( 3 ) , 'brain, hippocampus' ( 4 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 1 ) , brain ( 5 ) , COS (fibroblast) ( 4 ) , neuron-'brain, hippocampus' ( 1 , 4 )

Upstream Regulation
Regulatory protein:
RAC1 (human) ( 4 )
Putative in vivo kinases:
JNK1 (rat) ( 4 )
Kinases, in vitro:
JNK1 (human) ( 4 )
Treatments:
APV ( 4 ) , ascomycin ( 4 ) , bicuculline ( 4 ) , calphostin_C ( 4 ) , calyculin_A ( 4 ) , colforsin ( 4 ) , gly-aCSF ( 1 ) , KN-93 ( 4 ) , KT5720 ( 4 ) , long-term depression ( 4 ) , long-term_potentiation ( 4 ) , NBQX ( 4 ) , NMDA ( 1 , 4 ) , NSC23766 ( 4 ) , okadaic_acid ( 4 ) , PD98059 ( 4 ) , rolipram ( 4 ) , SB203580 ( 4 ) , SB600125 ( 4 ) , seliciclib ( 4 ) , TTX ( 4 )

Downstream Regulation
Effects of modification on PSD-95:
activity, induced ( 4 ) , intracellular localization ( 4 ) , protein stabilization ( 1 )

References 

1

Nelson CD, et al. (2013) Phosphorylation of Threonine-19 of PSD-95 by GSK-3β is Required for PSD-95 Mobilization and Long-Term Depression. J Neurosci 33, 12122-35
23864697   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Edbauer D, et al. (2009) Identification and characterization of neuronal mitogen-activated protein kinase substrates using a specific phosphomotif antibody. Mol Cell Proteomics 8, 681-95
19054758   Curated Info

4

Kim MJ, et al. (2007) Synaptic accumulation of PSD-95 and synaptic function regulated by phosphorylation of serine-295 of PSD-95. Neuron 56, 488-502
17988632   Curated Info

5

Jaffe H, Vinade L, Dosemeci A (2004) Identification of novel phosphorylation sites on postsynaptic density proteins. Biochem Biophys Res Commun 321, 210-8
15358237   Curated Info