Ser368
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Home > Phosphorylation Site Page: > Ser368  -  GJA1 (rat)

Site Information
QRPssRAssRAssRP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447813

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 15 ) , [32P] bio-synthetic labeling ( 14 , 15 ) , immunoprecipitation ( 3 , 4 , 13 ) , mass spectrometry ( 8 , 12 ) , mutation of modification site ( 3 , 9 , 11 , 13 , 14 ) , peptide sequencing ( 4 ) , phospho-antibody ( 6 , 9 , 10 , 13 , 16 ) , phosphoamino acid analysis ( 14 , 15 ) , phosphopeptide mapping ( 14 ) , western blotting ( 3 , 4 , 6 , 9 , 10 , 13 , 16 )
Relevant cell line - cell type - tissue:
'heart, embryo' ( 9 ) , fibroblast-skin ( 15 ) , granulosa ( 14 ) , heart ( 10 , 12 , 16 ) , HeLa (cervical) ( 3 , 14 ) , IEC-6 (epithelial) ( 8 ) , LA-25 (fibroblast) ( 6 ) , MDCK (epithelial) ( 3 ) , NRK (fibroblast) ( 4 ) , oocyte ( 13 ) , preovulatory follicle granulosa ( 11 ) , T51B (epithelial) ( 15 )

Upstream Regulation
Regulatory protein:
PKCA (rat) ( 13 )
Putative in vivo kinases:
PKACA (human) ( 11 ) , PKCA (mouse) ( 15 )
Kinases, in vitro:
PKACA (human) ( 11 ) , PKCA (cow) ( 15 ) , PKCA (human) ( 12 ) , PKCB (cow) ( 15 ) , PKCE (human) ( 12 ) , PKCG (human) ( 15 )
Putative upstream phosphatases:
PTPN2 (human) ( 6 )
Treatments:
angiotensin_2 ( 4 ) , calphostin_C ( 13 ) , DADLE ( 10 ) , FGF2 ( 9 , 16 ) , H-89 ( 11 ) , ischemia ( 9 , 10 , 12 ) , PD98059 ( 11 ) , phorbol_ester ( 5 , 7 , 15 ) , PKC-epsilon_translocation_inhibitor ( 10 ) , PKCE_inhibitor ( 7 ) , rotigaptide ( 12 ) , urofollitropin ( 11 , 14 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 14 ) , activity, inhibited ( 3 , 15 )

References 

1

Yang Y, et al. (2019) Connexin43 dephosphorylation at serine 282 is associated with connexin43-mediated cardiomyocyte apoptosis. Cell Death Differ
30770876   Curated Info

2

Bertholomey ML, et al. (2018) Phosphoproteomic Analysis of the Amygdala Response to Adolescent Glucocorticoid Exposure Reveals G-Protein Coupled Receptor Kinase 2 as a Target for Reducing Motivation for Alcohol. Proteomes 6
30322021   Curated Info

3

Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608
29021339   Curated Info

4

Li H, et al. (2016) Regulation of Connexin43 Function and Expression by Tyrosine Kinase 2. J Biol Chem 291, 15867-80
27235399   Curated Info

5

Kang M, et al. (2014) Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP3/IP3 receptor signaling. Cell Commun Signal 12, 58
25262337   Curated Info

6

Li H, et al. (2014) TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication. J Cell Sci 127, 3269-79
24849651   Curated Info

7

Srisakuldee W, et al. (2014) The FGF-2-triggered protection of cardiac subsarcolemmal mitochondria from calcium overload is mitochondrial connexin 43-dependent. Cardiovasc Res 103, 72-80
24654232   Curated Info

8

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

9

Srisakuldee W, et al. (2009) Phosphorylation of connexin-43 at serine 262 promotes a cardiac injury-resistant state. Cardiovasc Res 83, 672-81
19423616   Curated Info

10

Miura T, et al. (2007) Delta-opioid receptor activation before ischemia reduces gap junction permeability in ischemic myocardium by PKC-epsilon-mediated phosphorylation of connexin 43. Am J Physiol Heart Circ Physiol 293, H1425-31
17513490   Curated Info

11

Yogo K, et al. (2006) PKA implicated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells. J Reprod Dev 52, 321-8
16474210   Curated Info

12

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

13

Bao X, Altenberg GA, Reuss L (2004) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286, C647-54
14602580   Curated Info

14

Yogo K, et al. (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531, 132-6
12417300   Curated Info

15

Lampe PD, et al. (2000) Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication. J Cell Biol 149, 1503-12
10871288   Curated Info

16

Srisakuldee W, et al. Administration of FGF-2 to the heart stimulates connexin-43 phosphorylation at protein kinase C target sites. Cell Commun Adhes 13, 13-9
16613776   Curated Info