Tyr530
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Home > Phosphorylation Site Page: > Tyr530  -  Src (rat)

Site Information
FTSTEPQyQPGENL_   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448235

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 25 ) , immunoprecipitation ( 5 ) , mass spectrometry ( 2 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 22 , 23 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 10 , 17 , 18 , 19 , 20 , 21 , 24 , 25 ) , phosphoamino acid analysis ( 25 ) , phosphopeptide mapping ( 25 ) , western blotting ( 3 , 4 , 5 , 6 , 10 , 17 , 18 , 19 , 25 )
Disease tissue studied:
brain cancer ( 4 , 17 ) , glioma ( 4 , 17 )
Relevant cell line - cell type - tissue:
'brain, hippocampus, dentate gyrus' ( 6 ) , 'neuron, cortical'-brain ( 5 ) , A7r5 ('muscle, smooth') ( 24 ) , beta-pancreas ( 3 ) , brain ( 7 , 8 , 9 , 10 , 16 , 22 , 23 ) , C6 (glial) ( 4 , 17 ) , CHO-K1 (fibroblast) ( 17 ) , hepatocyte-liver ( 20 ) , intestine ( 19 ) , myocyte-heart ( 18 ) , PC CL 3 (thyroid cell) ( 21 ) , PC-12 (chromaffin) ( 11 , 12 , 13 , 14 , 15 ) , Rat2 (fibroblast) ( 25 ) , stomach ( 2 )

Upstream Regulation
Regulatory protein:
GJA1 (rat) ( 4 ) , SHP-2 (human) ( 17 )
Kinases, in vitro:
CSK (rat) ( 25 , 26 ) , Src (rat) ( 25 )
Putative upstream phosphatases:
PTPRA (rat) ( 21 )
Phosphatases, in vitro:
PTPRA (rat) ( 21 )
Treatments:
AG490 ( 17 ) , ATP ( 10 ) , BAPTA-AM ( 19 ) , BDNF ( 5 ) , BIM23926 ( 17 ) , cAMP_analog ( 3 ) , exenatide ( 3 ) , Fas ( 20 ) , ischemia/reperfusion ( 6 ) , neuregulin ( 18 ) , ouabain ( 24 ) , PP1 ( 5 ) , PTH ( 19 ) , PTX ( 17 ) , somatostatin ( 17 ) , SU6656 ( 6 ) , U0126 ( 6 ) , Zn(2+) ( 5 )

Downstream Regulation
Effects of modification on Src:
enzymatic activity, inhibited ( 21 ) , molecular association, regulation ( 21 )
Effects of modification on biological processes:
cell adhesion, altered ( 21 )
Induce interaction with:
PTPRA (rat) ( 21 )

References 

1

Xiong Y, et al. (2013) Zinc induces protein phosphatase 2A inactivation and tau hyperphosphorylation through Src dependent PP2A (tyrosine 307) phosphorylation. Neurobiol Aging 34, 745-56
22892311   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Mukai E, et al. (2011) Exendin-4 suppresses SRC activation and reactive oxygen species production in diabetic Goto-Kakizaki rat islets in an Epac-dependent manner. Diabetes 60, 218-26
20978090   Curated Info

4

Herrero-González S, et al. (2010) Connexin43 inhibits the oncogenic activity of c-Src in C6 glioma cells. Oncogene 29, 5712-23
20676131   Curated Info

5

Huang YZ, McNamara JO (2010) Mutual regulation of Src family kinases and the neurotrophin receptor TrkB. J Biol Chem 285, 8207-17
20064930   Curated Info

6

Tian HP, et al. (2009) Non-receptor tyrosine kinase Src is required for ischemia-stimulated neuronal cell proliferation via Raf/ERK/CREB activation in the dentate gyrus. BMC Neurosci 10, 139
19943942   Curated Info

7

Guo A (2008) CST Curation Set: 5216; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Guo A (2008) CST Curation Set: 5217; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Arachiche A, et al. (2008) Localization of PTP-1B, SHP-2, and Src exclusively in rat brain mitochondria and functional consequences. J Biol Chem 283, 24406-11
18583343   Curated Info

11

Guo A (2008) CST Curation Set: 3877; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Guo A (2008) CST Curation Set: 3878; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Guo A (2008) CST Curation Set: 3879; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Guo A (2008) CST Curation Set: 3880; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Stokes M (2007) CST Curation Set: 3525; Year: 2007; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

Guo A (2007) CST Curation Set: 2238; Year: 2007; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

17

Arena S, et al. (2007) An intracellular multi-effector complex mediates somatostatin receptor 1 activation of phospho-tyrosine phosphatase eta. Mol Endocrinol 21, 229-46
17021051   Curated Info

18

Kuramochi Y, Guo X, Sawyer DB (2006) Neuregulin activates erbB2-dependent src/FAK signaling and cytoskeletal remodeling in isolated adult rat cardiac myocytes. J Mol Cell Cardiol 41, 228-35
16769082   Curated Info

19

Gentili C, Boland R, Russo de Boland A (2006) Implication of Gbetagamma proteins and c-SRC tyrosine kinase in parathyroid hormone-induced signal transduction in rat enterocytes. J Endocrinol 188, 69-78
16394176   Curated Info

20

Reinehr R, et al. (2005) Involvement of NADPH oxidase isoforms and Src family kinases in CD95-dependent hepatocyte apoptosis. J Biol Chem 280, 27179-94
15917250   Curated Info

21

Pera IL, et al. (2005) The rat tyrosine phosphatase eta increases cell adhesion by activating c-Src through dephosphorylation of its inhibitory phosphotyrosine residue. Oncogene 24, 3187-95
15735685   Curated Info

22

Guo A (2005) CST Curation Set: 678; Year: 2005; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

23

Guo A (2005) CST Curation Set: 680; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

24

Haas M, Wang H, Tian J, Xie Z (2002) Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. J Biol Chem 277, 18694-702
11907028   Curated Info

25

MacAuley A, et al. (1993) Phosphorylation of Src mutants at Tyr 527 in fibroblasts does not correlate with in vitro phosphorylation by CSK. Oncogene 8, 117-24
7678701   Curated Info

26

Nada S, et al. (1991) Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src. Nature 351, 69-72
1709258   Curated Info