Ser244
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Home > Phosphorylation Site Page: > Ser244  -  S6 (rat)

Site Information
LRAstsKsESsQK__   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448095

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 7 ) , [32P] bio-synthetic labeling ( 7 ) , mass spectrometry ( 1 , 2 ) , peptide sequencing ( 7 ) , phospho-antibody ( 3 , 4 , 5 ) , phosphoamino acid analysis ( 7 ) , phosphopeptide mapping ( 7 ) , western blotting ( 3 )
Disease tissue studied:
adrenal cancer ( 2 ) , pheochromocytoma ( 2 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 4 , 5 ) , intestine ( 1 ) , L6 (myoblast) ( 3 ) , liver ( 1 , 7 ) , lung ( 1 ) , pancreas ( 1 ) , PC-12 (chromaffin) ( 2 ) , stomach ( 1 ) , thymus ( 1 )

Upstream Regulation
Kinases, in vitro:
p70S6K (rat) ( 6 )
Treatments:
API-2 ( 3 ) , cycloheximide ( 7 ) , exercise ( 4 , 5 ) , insulin ( 3 ) , PIK90 ( 3 ) , PP242 ( 3 ) , rapamycin ( 3 , 4 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Zhou J (2009) CST Curation Set: 6820; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

3

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

4

Kubica N, et al. (2005) Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner. J Biol Chem 280, 7570-80
15591312   Curated Info

5

Bolster DR, et al. (2003) Immediate response of mammalian target of rapamycin (mTOR)-mediated signalling following acute resistance exercise in rat skeletal muscle. J Physiol 553, 213-20
12937293   Curated Info

6

Ferrari S, et al. (1991) Mitogen-activated 70K S6 kinase. Identification of in vitro 40 S ribosomal S6 phosphorylation sites. J Biol Chem 266, 22770-5
1939282   Curated Info

7

Krieg J, Hofsteenge J, Thomas G (1988) Identification of the 40 S ribosomal protein S6 phosphorylation sites induced by cycloheximide. J Biol Chem 263, 11473-7
3403539   Curated Info