Ser240
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Home > Phosphorylation Site Page: > Ser240  -  S6 (rat)

Site Information
RLssLRAstsKsESs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448094

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 11 ) , [32P] bio-synthetic labeling ( 11 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 ) , peptide sequencing ( 11 ) , phospho-antibody ( 7 , 8 , 9 ) , phosphoamino acid analysis ( 11 ) , phosphopeptide mapping ( 11 ) , western blotting ( 7 )
Disease tissue studied:
adrenal cancer ( 3 , 4 , 5 , 6 ) , pheochromocytoma ( 3 , 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 1 ) , 'brain, cerebellum' ( 1 ) , 'brain, cerebral cortex' ( 1 ) , 'muscle, skeletal' ( 8 , 9 ) , adipose tissue ( 1 ) , heart ( 1 ) , intestine ( 1 ) , kidney ( 1 ) , L6 (myoblast) ( 7 ) , liver ( 1 , 2 , 11 ) , lung ( 1 ) , muscle ( 1 ) , pancreas ( 1 ) , PC-12 (chromaffin) ( 3 , 4 , 5 , 6 ) , spleen ( 1 ) , stomach ( 1 ) , testis ( 1 ) , thymus ( 1 )

Upstream Regulation
Kinases, in vitro:
p70S6K (rat) ( 10 ) , PKACA (cow) ( 12 )
Treatments:
API-2 ( 7 ) , cycloheximide ( 11 ) , exercise ( 8 , 9 ) , insulin ( 7 ) , PIK90 ( 7 ) , PP242 ( 7 ) , rapamycin ( 2 , 7 , 8 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

3

Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

4

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

5

Zhou J (2009) CST Curation Set: 6821; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

6

Zhou J (2009) CST Curation Set: 6820; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

7

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

8

Kubica N, et al. (2005) Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner. J Biol Chem 280, 7570-80
15591312   Curated Info

9

Bolster DR, et al. (2003) Immediate response of mammalian target of rapamycin (mTOR)-mediated signalling following acute resistance exercise in rat skeletal muscle. J Physiol 553, 213-20
12937293   Curated Info

10

Ferrari S, et al. (1991) Mitogen-activated 70K S6 kinase. Identification of in vitro 40 S ribosomal S6 phosphorylation sites. J Biol Chem 266, 22770-5
1939282   Curated Info

11

Krieg J, Hofsteenge J, Thomas G (1988) Identification of the 40 S ribosomal protein S6 phosphorylation sites induced by cycloheximide. J Biol Chem 263, 11473-7
3403539   Curated Info

12

Wettenhall RE, Morgan FJ (1984) Phosphorylation of hepatic ribosomal protein S6 on 80 and 40 S ribosomes. Primary structure of S6 in the region of the major phosphorylation sites for cAMP-dependent protein kinases. J Biol Chem 259, 2084-91
6698958   Curated Info