Ser235
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Home > Phosphorylation Site Page: > Ser235  -  S6 (rat)

Site Information
IAKRRRLssLRAsts   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448092

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 5 , 26 ) , [32P] bio-synthetic labeling ( 26 , 27 ) , immunoassay ( 2 ) , mass spectrometry ( 3 , 7 , 8 , 9 , 10 ) , mutation of modification site ( 12 ) , peptide sequencing ( 26 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ) , phosphoamino acid analysis ( 26 ) , phosphopeptide mapping ( 26 , 27 ) , western blotting ( 1 , 2 , 4 , 5 , 6 , 11 , 12 , 13 , 14 , 15 , 19 , 20 , 22 , 23 )
Disease tissue studied:
adrenal cancer ( 7 , 8 , 9 , 10 ) , pheochromocytoma ( 7 , 8 , 9 , 10 ) , kidney cancer ( 6 , 20 ) , pancreatic cancer ( 2 ) , pancreatic carcinoma ( 2 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 4 , 14 , 15 , 16 , 18 , 21 , 22 ) , CACL1-111 (renal) ( 20 ) , CHO-K1 (fibroblast) ( 23 ) , COS (fibroblast) ( 12 ) , ELT-3 ( 6 ) , HEK293T (epithelial) ( 12 ) , hepatocyte-liver ( 27 ) , IEC-18 (epithelial) ( 19 ) , INS-1 (pancreatic) ( 2 ) , kidney ( 6 ) , L6 (myoblast) ( 11 ) , liver ( 3 , 26 ) , MKOC1 (renal) ( 20 ) , myocyte-heart ( 13 ) , neuron-'brain, cerebral cortex' ( 12 ) , neuron-'brain, hippocampus' ( 1 ) , NR-22 (fibroblast) ( 20 ) , pancreatic islet ( 2 ) , PC CL 3 (thyroid cell) ( 5 ) , PC-12 (chromaffin) ( 7 , 8 , 9 , 10 ) , sertoli-testis ( 17 )

Upstream Regulation
Regulatory protein:
CAB39 (human) ( 13 ) , CDK5 (rat) ( 12 ) , LKB1 (mouse) ( 13 ) , STRAD (human) ( 13 )
Putative in vivo kinases:
Akt1 (rat) ( 19 ) , Akt2 (rat) ( 19 )
Kinases, in vitro:
p70S6K (rat) ( 25 ) , PKACA (cow) ( 27 )
Treatments:
acetominophen ( 4 ) , bicuculline ( 1 ) , cAMP_analog ( 17 ) , colforsin ( 2 , 17 ) , cycloheximide ( 26 ) , development ( 4 , 15 ) , DMOG ( 2 ) , exercise ( 18 , 21 ) , FTI277 ( 17 ) , gabazine ( 1 ) , GLP-1 ( 2 ) , H-89 ( 17 ) , H2O2 ( 2 ) , IGF-1 ( 14 ) , indinavir ( 16 ) , insulin ( 5 , 11 , 16 , 17 , 23 ) , Intralipid ( 14 ) , leucine ( 14 ) , lithium ( 23 ) , LY294002 ( 2 , 17 ) , mechanical stress ( 15 ) , PP242 ( 2 , 11 ) , rapamycin ( 2 , 3 , 11 , 17 , 18 , 20 ) , SB415286 ( 23 ) , TSH ( 5 ) , urofollitropin ( 17 ) , wortmannin ( 17 )

Downstream Regulation
Effects of modification on biological processes:
signaling pathway regulation ( 2 )

References 

1

Srivastava T, et al. (2012) Regulation of neuronal mRNA translation by CaM-kinase I phosphorylation of eIF4GII. J Neurosci 32, 5620-30
22514323   Curated Info

2

Van de Velde S, Hogan MF, Montminy M (2011) mTOR links incretin signaling to HIF induction in pancreatic beta cells. Proc Natl Acad Sci U S A 108, 16876-82
21949366   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Wu M, et al. (2010) Acetaminophen improves protein translational signaling in aged skeletal muscle. Rejuvenation Res 13, 571-9
20818934   Curated Info

5

Blancquaert S, et al. (2010) cAMP-dependent activation of mammalian target of rapamycin (mTOR) in thyroid cells. Implication in mitogenesis and activation of CDK4. Mol Endocrinol 24, 1453-68
20484410   Curated Info

6

Short JD, et al. (2010) AMPK-mediated phosphorylation of murine p27 at T197 promotes binding of 14-3-3 proteins and increases p27 stability. Mol Carcinog 49, 429-39
20146253   Curated Info

7

Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

8

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

9

Zhou J (2009) CST Curation Set: 6821; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

10

Zhou J (2009) CST Curation Set: 6820; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

11

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

12

Hou Z, He L, Qi RZ (2007) Regulation of s6 kinase 1 activation by phosphorylation at ser-411. J Biol Chem 282, 6922-8
17220300   Curated Info

13

Noga AA, et al. (2007) Expression of an active LKB1 complex in cardiac myocytes results in decreased protein synthesis associated with phenylephrine-induced hypertrophy. Am J Physiol Heart Circ Physiol 292, H1460-9
17098823   Curated Info

14

Lang CH (2006) Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle. Am J Physiol Endocrinol Metab 291, E666-74
16684854   Curated Info

15

Thomson DM, Gordon SE (2006) Impaired overload-induced muscle growth is associated with diminished translational signalling in aged rat fast-twitch skeletal muscle. J Physiol 574, 291-305
16627569   Curated Info

16

Hong-Brown LQ, et al. (2005) Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle. Am J Physiol Endocrinol Metab 289, E382-90
15827064   Curated Info

17

L├ęcureuil C, et al. (2005) Follicle-stimulating hormone activates p70 ribosomal protein S6 kinase by protein kinase A-mediated dephosphorylation of Thr 421/Ser 424 in primary Sertoli cells. Mol Endocrinol 19, 1812-20
15774499   Curated Info

18

Kubica N, et al. (2005) Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner. J Biol Chem 280, 7570-80
15591312   Curated Info

19

Chiu T, Santiskulvong C, Rozengurt E (2005) EGF receptor transactivation mediates ANG II-stimulated mitogenesis in intestinal epithelial cells through the PI3-kinase/Akt/mTOR/p70S6K1 signaling pathway. Am J Physiol Gastrointest Liver Physiol 288, G182-94
15358595   Curated Info

20

Adachi H, et al. (2004) Niban gene is commonly expressed in the renal tumors: a new candidate marker for renal carcinogenesis. Oncogene 23, 3495-500
14990989   Curated Info

21

Bolster DR, et al. (2003) Immediate response of mammalian target of rapamycin (mTOR)-mediated signalling following acute resistance exercise in rat skeletal muscle. J Physiol 553, 213-20
12937293   Curated Info

22

Kumar V, Frost RA, Lang CH (2002) Alcohol impairs insulin and IGF-I stimulation of S6K1 but not 4E-BP1 in skeletal muscle. Am J Physiol Endocrinol Metab 283, E917-28
12376318   Curated Info

23

Wang X, et al. (2002) Evidence that the dephosphorylation of Ser(535) in the epsilon-subunit of eukaryotic initiation factor (eIF) 2B is insufficient for the activation of eIF2B by insulin. Biochem J 367, 475-81
12133000   Curated Info

24

Flotow H, Thomas G (1992) Substrate recognition determinants of the mitogen-activated 70K S6 kinase from rat liver. J Biol Chem 267, 3074-8
1737763   Curated Info

25

Ferrari S, et al. (1991) Mitogen-activated 70K S6 kinase. Identification of in vitro 40 S ribosomal S6 phosphorylation sites. J Biol Chem 266, 22770-5
1939282   Curated Info

26

Krieg J, Hofsteenge J, Thomas G (1988) Identification of the 40 S ribosomal protein S6 phosphorylation sites induced by cycloheximide. J Biol Chem 263, 11473-7
3403539   Curated Info

27

Wettenhall RE, Morgan FJ (1984) Phosphorylation of hepatic ribosomal protein S6 on 80 and 40 S ribosomes. Primary structure of S6 in the region of the major phosphorylation sites for cAMP-dependent protein kinases. J Biol Chem 259, 2084-91
6698958   Curated Info