Thr56
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Home > Phosphorylation Site Page: > Thr56  -  Bcl-2 (human)

Site Information
FSSQPGHtPHPAASR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448655

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , electrophoretic mobility shift ( 9 , 10 ) , immunoprecipitation ( 3 , 6 , 10 ) , mass spectrometry ( 1 ) , mutation of modification site ( 5 , 7 , 8 , 9 , 10 , 11 , 12 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 11 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 11 , 12 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , colorectal cancer ( 4 ) , colorectal carcinoma ( 4 ) , leukemia ( 10 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 ) , non-small cell squamous cell lung carcinoma ( 4 ) , neuroblastoma ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
JNK3 (mouse) ( 12 ) , LKB1 (human) ( 4 ) , RAC1 (human) ( 12 )
Putative in vivo kinases:
CDK1 (human) ( 10 ) , ERK2 (human) ( 11 ) , P38A (human) ( 3 , 5 )
Kinases, in vitro:
CDK1 (human) ( 10 ) , ERK1 (human) ( 11 ) , JNK3 (mouse) ( 12 ) , P38A (human) ( 5 )
Treatments:
ABT-737 ( 2 ) , K252a ( 5 ) , okadaic_acid ( 10 ) , SB203580 ( 3 , 5 ) , siRNA ( 4 ) , virus infection ( 3 )

Downstream Regulation
Effects of modification on Bcl-2:
molecular association, regulation ( 6 ) , protein stabilization ( 11 ) , ubiquitination ( 11 )
Effects of modification on biological processes:
apoptosis, altered ( 3 , 4 , 9 ) , apoptosis, induced ( 5 , 8 , 11 ) , apoptosis, inhibited ( 7 ) , cell cycle regulation ( 10 )
Induce interaction with:
CDK1 (human) ( 6 ) , NCL (human) ( 6 ) , PPP1CA (human) ( 6 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Beurlet S, et al. (2013) BCL-2 inhibition with ABT-737 prolongs survival in an NRAS/BCL-2 mouse model of AML by targeting primitive LSK and progenitor cells. Blood 122, 2864-76
23943652   Curated Info

3

Nencioni L, et al. (2009) Bcl-2 Expression and p38MAPK Activity in Cells Infected with Influenza A Virus: IMPACT ON VIRALLY INDUCED APOPTOSIS AND VIRAL REPLICATION. J Biol Chem 284, 16004-15
19336399   Curated Info

4

Zhong D, et al. (2008) LKB1 is necessary for Akt-mediated phosphorylation of proapoptotic proteins. Cancer Res 68, 7270-7
18794113   Curated Info

5

De Chiara G, et al. (2006) Bcl-2 Phosphorylation by p38 MAPK: identification of target sites and biologic consequences. J Biol Chem 281, 21353-61
16714293   Curated Info

6

Barboule N, Truchet I, Valette A (2005) Localization of phosphorylated forms of Bcl-2 in mitosis: co-localization with Ki-67 and nucleolin in nuclear structures and on mitotic chromosomes. Cell Cycle 4, 590-6
15876860   Curated Info

7

Huang ST, Cidlowski JA (2002) Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes. FASEB J 16, 825-32
12039864   Curated Info

8

Torcia M, et al. (2001) Nerve growth factor inhibits apoptosis in memory B lymphocytes via inactivation of p38 MAPK, prevention of Bcl-2 phosphorylation, and cytochrome c release. J Biol Chem 276, 39027-36
11495898   Curated Info

9

Thomas A, Giesler T, White E (2000) p53 mediates bcl-2 phosphorylation and apoptosis via activation of the Cdc42/JNK1 pathway. Oncogene 19, 5259-69
11077443   Curated Info

10

Furukawa Y, et al. (2000) Phosphorylation of Bcl-2 protein by CDC2 kinase during G2/M phases and its role in cell cycle regulation. J Biol Chem 275, 21661-7
10766756   Curated Info

11

Breitschopf K, et al. (2000) Posttranslational modification of Bcl-2 facilitates its proteasome-dependent degradation: molecular characterization of the involved signaling pathway. Mol Cell Biol 20, 1886-96
10669763   Curated Info

12

Maundrell K, et al. (1997) Bcl-2 undergoes phosphorylation by c-Jun N-terminal kinase/stress-activated protein kinases in the presence of the constitutively active GTP-binding protein Rac1. J Biol Chem 272, 25238-42
9312139   Curated Info