Ser370
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Home > Phosphorylation Site Page: > Ser370  -  PTEN (human)

Site Information
TSVtPDVsDNEPDHy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448649

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 12 , 13 , 14 ) , immunoprecipitation ( 1 , 5 ) , mass spectrometry ( 2 , 4 , 12 ) , mass spectrometry (in vitro) ( 7 ) , mutation of modification site ( 1 , 3 , 7 , 10 , 12 , 13 , 14 ) , phospho-antibody ( 5 , 7 , 8 , 9 , 10 ) , phosphoamino acid analysis ( 12 , 14 ) , phosphopeptide mapping ( 10 ) , western blotting ( 1 , 5 , 7 , 8 , 9 , 10 )
Disease tissue studied:
brain cancer ( 12 ) , glioblastoma ( 12 ) , glioma ( 12 ) , breast cancer ( 2 , 8 ) , colorectal cancer ( 9 ) , colorectal carcinoma ( 9 ) , pancreatic cancer ( 8 ) , pancreatic carcinoma ( 8 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CK2A1 (human) ( 9 ) , PLK3 (mouse) ( 7 )
Kinases, in vitro:
CK2A1 (human) ( 11 , 12 , 13 ) , PLK3 (human) ( 7 )
Treatments:
adriamycin ( 5 ) , CK2_inhibitor ( 9 ) , D4476 ( 5 ) , etoposide ( 5 ) , GSK-3_Inhibitor_XVI ( 5 ) , H2O2 ( 5 ) , silmitasertib ( 8 ) , TBB ( 5 )

Downstream Regulation
Effects of modification on PTEN:
enzymatic activity, inhibited ( 10 ) , intracellular localization ( 5 ) , molecular association, regulation ( 5 ) , protein stabilization ( 13 )
Induce interaction with:
RAD52 (human) ( 5 )
Inhibit interaction with:
PTEN (human) ( 10 )

References 

1

Yang JM, et al. (2017) Characterization of PTEN mutations in brain cancer reveals that pten mono-ubiquitination promotes protein stability and nuclear localization. Oncogene 36, 3673-3685
28263967   Curated Info

2

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

3

Choi BH, Pagano M, Dai W (2014) Plk1 Protein Phosphorylates Phosphatase and Tensin Homolog (PTEN) and Regulates Its Mitotic Activity during the Cell Cycle. J Biol Chem 289, 14066-74
24706748   Curated Info

4

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

5

Choi BH, Chen Y, Dai W (2013) Chromatin PTEN is involved in DNA damage response partly through regulating Rad52 sumoylation. Cell Cycle 12, 3442-7
24047694   Curated Info

6

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

7

Xu D, et al. (2010) Regulation of PTEN stability and activity by Plk3. J Biol Chem 285, 39935-42
20940307   Curated Info

8

Siddiqui-Jain A, et al. (2010) CX-4945, an orally bioavailable selective inhibitor of protein kinase CK2, inhibits prosurvival and angiogenic signaling and exhibits antitumor efficacy. Cancer Res 70, 10288-98
21159648   Curated Info

9

Zhu D, et al. (2010) Inhibition of protein kinase CK2 expression and activity blocks tumor cell growth. Mol Cell Biochem 333, 159-67
19629644   Curated Info

10

Odriozola L, Singh G, Hoang T, Chan AM (2007) Regulation of PTEN activity by its carboxyl-terminal autoinhibitory domain. J Biol Chem 282, 23306-15
17565999   Curated Info

11

Al-Khouri AM, et al. (2005) Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta. J Biol Chem 280, 35195-202
16107342   Curated Info

12

Miller SJ, et al. (2002) Direct identification of PTEN phosphorylation sites. FEBS Lett 528, 145-53
12297295   Curated Info

13

Torres J, Pulido R (2001) The tumor suppressor PTEN is phosphorylated by the protein kinase CK2 at its C terminus. Implications for PTEN stability to proteasome-mediated degradation. J Biol Chem 276, 993-8
11035045   Curated Info

14

Vazquez F, Ramaswamy S, Nakamura N, Sellers WR (2000) Phosphorylation of the PTEN tail regulates protein stability and function. Mol Cell Biol 20, 5010-8
10866658   Curated Info